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Western blot analysis of ATOH8 in A-20 cell lysate with ATOH8 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of ATOH8 in mouse brain tissue with ATOH8 antibody at 5 μg/mL.
Western blot analysis of ATOH8 in A-20 cell lysate with ATOH8 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

ATOH8 Antibody

CATALOG NUMBER: 5109

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
ATOH8 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ATOH8.
The immunogen is located within the last 50 amino acids of ATOH8.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
ATOH8 antibody can be used for detection of ATOH8 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Predicted Molecular Weight:
Predicted: 35 kDa
Observed: 40 kDa
Purification:
ATOH8 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ATOH8 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ATOH8 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ATOH8
Additional Names:
ATOH8 Antibody: HATH6, bHLHa21, ATH6, BHLHA21, Protein atonal homolog 8, Class A basic helix-loop-helix protein 21
Protein Accession Number:
NP_116216
Protein Gi Number:
226498192
Ncbi Gene Id Number:
84913
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ATOH8 Antibody: Basic helix-loop-helix (bHLH) transcription factors play important roles in differentiation processes during embryonic development of vertebrates. ATOH8 (MATH6) is a tissue-restricted member of the atonal superfamily of bHLH transcription factors that exhibits 43-57% identity in the bHLH domain with other mammalian atonal paralogs including the NeuroD and Neurogenin factors. In the mouse, ATOH8 has been implicated in the specification and differentiation of neuronal cell lineages in the brain and may also participate in kidney development. Recent studies show that ATOH8 is a novel component of the pancreatic transcriptional network during embryonic development and suggest a potential role as a modulator of the differentiation program initiated by the pro-endocrine factor Neurog3. It is indispensable for early embryonic development, suggesting a more widespread function for this factor in tissue-specific differentiation processes that are dependent on class II bHLH genes.
Background Reference 1:
Wasserman SM, Mehraban F, Komuves LG, et al. Gene expression profile of human endothelial cells exposed to sustained fluid shear stress. Physiol. Genomics 2002;12:13-23.
Background Reference 2:
Ledent V and Vervoort M. The basic helix-loop-helix protein family: comparative genomics and phylogenetic analysis. Genome Res. 2001; 11:754-770.
Background Reference 3:
Inoue C, Bae SK, Takatsuka K, et al. Math6, a bHLH gene expressed in the developing nervous system, regulates neuronal versus glial differentiation. Genes Cells 2001; 6:977-986.
Background Reference 4:
Ross MD, Martinka S, Mukherjee A, et al. Math6 expression during kidney development and altered expression in a mouse model of glomerulosclerosis. Dev Dyn. 2006; 235:3102-3109.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5109

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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