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Western blot analysis of FTO in human uterus tissue lysate with FTO antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of FTO in mouse brain tissue with FTO antibody at 2.5 μg/mL.
Immunofluorescence of FTO in Mouse Brain cells with FTO antibody at 20 μg/mL.
Western blot analysis of FTO in human uterus tissue lysate with FTO antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

FTO Antibody

CATALOG NUMBER: 5137

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%), Mouse: (100%)
Immunogen:
FTO antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human FTO.
The immunogen is located within the first 50 amino acids of FTO.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
FTO antibody can be used for detection of FTO by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1317 - Human Uterus Tissue Lysate
Purification:
FTO Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
FTO Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
FTO antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FTO
Additional Names:
FTO Antibody: ALKBH9, KIAA1752, Alpha-ketoglutarate-dependent dioxygenase FTO, Fat mass and obesity-associated protein
Protein Accession Number:
Q9C0B1
Protein Gi Number:
148841515
Ncbi Gene Id Number:
79068
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FTO Antibody: Rising obesity rates are rapidly becoming a growing health concern in the developing world. The fat mass and obesity associated gene (FTO) is the first gene discovered to contribute to common forms of human obesity. FTO is a member of the non-heme dioxygenase superfamily, encoding a 2-oxoglutarate-dependent nucleic acid demethylase whose mRNA is widely expressed, especially in neurons of feeding-related nuclei of the brain. FTO mRNA in the arcuate nucleus in mice is up-regulated by feeding and down-regulated during fasting, although the opposite pattern has been observed in rats. At least four isoforms of FTO are known to exist.
Background Reference 1:
Scuteri A, Sanna S, Chen W-M, et al. Genome-wide association scan shows genetic variants in the FTO gene are associated with obesity-related traits. PloS Genet.2007; 3:e115.
Background Reference 2:
Gerken T, Girard CA, Tung YCL, et al. The obesity-associated FTO gene encodes a 2-oxyglutarate-dependent nucleic acid demethylase. Science2007; 318:1469-72.
Background Reference 3:
Fredriksson R, Hagglund M, Olszewski PK, et al. The obesity gene, FTO, is of ancient origin, upregulated during food deprivation and expressed in neurons of feeding-related nuclei of the brain. Endocrinology2008; 149:2062-71.
Background Reference 4:
Stratigopoulous G, Padilla S, Leduc CA, et al. Regulation of FTO/FTM gene expression in mice and humans. Am. J. Physiol. Regul. Integr. Comp. Physiol.2008; 294:R1185-96.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5137

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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