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<strong>Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: CLDN1, 5187 (1 μg/mL), CLDN1, 24-027 (2 μg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation in Human HepG2 Cell Lysate</strong><br>Loading: 15 μg of lysates per lane.Antibodies: CLDN1, 5187 (A: 1 μg/mL and B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Human Tissue Lysates</strong><br>Loading: 15 μg of lysates per lane.Antibodies: CLDN1, 5187 (1 μg/mL) , 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Human Tissue Lysates</strong><br>Loading: 15 μg of lysates per lane.Antibodies: CLDN1, 5187 (1 μg/mL) , 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 Immunofluorescence Validation of CLDN1 in HepG2 Cells</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling CLDN1 with 5187 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: CLDN1, 5187 (1 μg/mL), CLDN1, 24-027 (2 μg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
IAV
Trial Size

CLDN1 Antibody

CATALOG NUMBER: 5187

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Mouse (95%)
Immunogen:
Anti-CLDN1 antibody (5187) was raised against a peptide corresponding to 20 amino acids near the carboxy terminus of human CLDN1.
The immunogen is located within the last 50 amino acids of CLDN1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
WB: 1-2 μg/mL; ICC: 5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and rat samples; Immunocytochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 2:
Cat. No. 17-011 - Hep G2 Cell Slide
Predicted Molecular Weight:
Predicted: 23kD
Observed: 23 kD
Validation:

Independent Antibody Validation in Cell lines (Figure 1) shows similar CLDN1 expression profile in human cell lines detected by two independent anti-CLDN1 antibodies that recognize different epitopes, 5187 against C-terminus domain and 24-027 against recombinant fusion protein.  CLDN1 proteins are detected in some tested cell lines at different expression levels by the two independent antibodies. 

Isoforms:
Human CLDN1 has one isoform (211aa, 23kD). Mouse CLDN1 has one isoform (211aa, 23kD) and Rat CLDN1 also has one isoform (211aa, 23kD). 5187 can detect human, mouse and rat.
Purification:
CLDN1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CLDN1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CLDN1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CLDN1
Additional Names:
CLDN1 Antibody: CLD1, SEMP1, ILVASC, CLD1, UNQ481/PRO944, Claudin-1, Senescence-associated epithelial membrane protein
Protein Accession Number:
CAG33419
Protein Gi Number:
48146393
Ncbi Gene Id Number:
9076
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CLDN1 Antibody: Claudin1 (CLDN1), a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Tight junctions are specialized regions of cell to cell contact consisting of networking strands that act as a molecular gasket for preventing the leakage of ions, water, etc., between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition and CLDN1 expression is often cell type and tissue dependent. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. CLDN1 and CLDN2 were found to be overexpressed in colonal cancer tissues and may be useful as tumor markers and targets for the treatment of colorectal cancer. Characterization of Claudins expression in human tumors can be an additional diagnostic tool. Recent studies show that CLDN1 has gastric tumor suppressive activity and is a direct transcriptional target of RUNX3. Along with SCARB1, LDL-R, and the tetraspanin superfamily member CD81, CLDN1 has been reported to be an entry factor for the Hepatitis C virus.
Background Reference 1:
Tepass. Nat. Cell Biol.2003; 5:595-7.
Background Reference 2:
Kinugasa et al. Anticancer Res.2007; 27:3729-34.
Background Reference 3:
Kinugasa et al. Anticancer Res.2007; 27:3729-34.Mod. Pathol.2007; 20:947-954.
Background Reference 4:
Morohashi et al. Int. J. Mol. Med.2007; 20:139-43.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5187

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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