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Western blot analysis of ZCRB1 in Raji cell lysate with ZCRB1 antibody at (A) 1 and (B) 2 μg/mL.
Western blot analysis of ZCRB1 in Raji cell lysate with ZCRB1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

ZCRB1 Antibody

CATALOG NUMBER: 5225

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
ZCRB1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human ZCRB1.
The immunogen is located within the last 50 amino acids of ZCRB1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ZCRB1 antibody can be used for detection of ZCRB1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1207 - Raji Cell Lysate
Purification:
ZCRB1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZCRB1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZCRB1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZCRB1
Additional Names:
ZCRB1 Antibody: MADP1, RBM36, MADP-1, SNRNP31, ZCCHC19, Zinc finger CCHC-type and RNA-binding motif-containing protein 1
Protein Accession Number:
NP_149105
Protein Gi Number:
51243065
Ncbi Gene Id Number:
85437
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZCRB1 Antibody: ZCRB1 is a nuclear protein first identified in a differential display screen involving morphine-dependence-related genes. Its expression is increased following morphine treatment and has been observed to be elevated in HepG2 cells. ZCRB1 contains a CCHC-type zing finger RNA-binding motif and can interact with the DBA-binding domain of the stem cell regulator C/EBP transcription factors. ZCRB1 was identified as one of the protein components of U11/U12 snRNPs, which are components of U12-type spliceosome and function as a molecular bridge connecting both ends of the intron, suggesting ZCRB1 may play a key role in U12-type splicing.
Background Reference 1:
Wang H, Gao MX, Li L, et al. Isolation, expression, and characterization of the human ZCRB1 gene mapped to 12q2. Genomics2007; 89:59-69.
Background Reference 2:
Trivedi AK, Bararia D, Christopeit M, et al. Proteomic identification of C/EBP-DBD multiprotein complex: JNK1 activates stem cell regulator C/EBPalpha by inhibiting its ubiquitination. Oncogene2007; 1789-801.
Background Reference 3:
Will CL, Schneider C, Hossbach M, et al. The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome. RNA2004; 10:929-41.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5225

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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