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Western blot analysis of CIP75 in 3T3 cell lysate with CIP75 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of CIP75 in mouse kidney tissue with CIP75 antibody at 5 μg/mL.
Western blot analysis of CIP75 in 3T3 cell lysate with CIP75 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

CIP75 Antibody

CATALOG NUMBER: 5267

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
CIP75 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human CIP75.
The immunogen is located within the first 50 amino acids of CIP75.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
CIP75 antibody can be used for detection of CIP75 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Purification:
CIP75 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CIP75 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CIP75 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
UBQLN4
Additional Names:
CIP75 Antibody: A1U, A1Up, UBIN, CIP75, C1orf6, Ubiquilin-4, Ataxin-1 interacting ubiquitin-like protein
Protein Accession Number:
Q9NRR5
Protein Gi Number:
45476982
Ncbi Gene Id Number:
56893
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CIP75 Antibody: The ubiquitin-proteosome pathway of protein degradation is one of the mechanisms that ensure the proper level of cellular proteins. The ubiquitin-like (UbL) and ubiquitin-associated (UBA) domain containing protein family is thought to be involved in proteosomal degradation. One such protein is CIP75, also known as ubiquilin-4, interacts with a number of proteins such as Ataxin-1 and Connexin-43, resulting in an increased rate of turnover of these proteins. Overexpression CIP75 led to a significant reduction of Connexin-43 half-life, with the opposite being observed in siRNA knockdown experiments. CIP75 contains an N-terminal UbL domain which is thought to interact with proteins of the 26S proteosome complex and a C-terminal UBA domain which appears to mediate its interaction with Connexin-75. At least three isoforms of CIP75 are known to exist.
Background Reference 1:
Su V and Lau AF. Ubiquitin-like and ubiquitin-associated domain proteins: significance in proteasomal degradation. Cell. Mol. Life Sci.2009; 66:2819-33.
Background Reference 2:
Davidson JD, Riley B, Burright EN, et al. Identification and characterization of an ataxin-1-interacting protein: A1Up, a ubiquitin-like protein. Hum. Mol. Genet.2000; 9:2305-12.
Background Reference 3:
Li X, Su V, Kurata WE, et al. A novel Connexin43-interacting protein, CIP75, which belongs to the UbL-UBA protein family, regulates the turnover of Connexin4 J. Biol. Chem.2008; 283:5748-59.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5267

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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