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Western blot analysis of TWA1 in human brain tissue lysate with TWA1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of TWA1 in rat brain tissue with TWA1 antibody at 2.5 μg/mL.
Immunofluorescence of TWA1 in rat brain tissue with TWA1 antibody at 20 μg/mL.
Western blot analysis of TWA1 in human brain tissue lysate with TWA1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

TWA1 Antibody

CATALOG NUMBER: 5305

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (100%), Bovine: (100%)
Immunogen:
TWA1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human TWA1.
The immunogen is located within the last 50 amino acids of TWA1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TWA1 antibody can be used for detection of TWA1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Purification:
TWA1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TWA1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TWA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GID8
Additional Names:
TWA1 Antibody: TWA1, C20orf11, Glucose-induced degradation protein 8 homolog, Two hybrid-associated protein 1 with RanBPM, Twa1
Protein Accession Number:
NP_060366
Protein Gi Number:
8923557
Ncbi Gene Id Number:
54994
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TWA1 Antibody: TWA1 was identified through a two hybrid-associated protein screen with RanBPM. TWA1 is well conserved through evolution and is localized within the nucleus. It interacts with RanBP9 and comprises a protein complex with RanBPM and Muskelin. TWA1 was found to possess the LisH-CTLH motif which is detected in proteins involved in microtubule dynamics, cell migration, nucleokinesis and chromosome segregation. These functions overlap with functions suggested for the RanGTPase cycle. Recent findings suggested that there is an as yet uncovered function of the RanGTPase cycle.
Background Reference 1:
Umeda M, Nishitani H, and Nishimoto T. A novel nuclear protein, Twa1, and Muskelin comprise a complex with RanBPM. Gene2003; 303:47-54.
Background Reference 2:
Kobayashi N, Yang J, Ueda A, et al. RanBPM, Muskelin, p48EMLP, p44CTLH, and the armadillo-repeat proteins ARMC8alpha and ARMC8beta are components of the CTLH complex. Gene2007; 396:236-47.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5305

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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