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Western blot analysis of JMJD1B in rat liver tissue lysate with JMJD1B antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of JMJD1B in rat liver tissue with JMJD1B antibody at 2.5 μg/mL.
Immunofluorescence of JMJD1B in rat liver tissue with JMJD1B antibody at 20 μg/mL.
Western blot analysis of JMJD1B in rat liver tissue lysate with JMJD1B antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

JMJD1B Antibody

CATALOG NUMBER: 5369

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
JMJD1B antibody was raised against a 20 amino acid synthetic peptide from near the center of human JMJD1B.
The immunogen is located within amino acids 1050 - 1100 of JMJD1B.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
JMJD1B antibody can be used for detection of JMJD1B by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificity:
This antibody will not cross-react with JMJD1A or JMJD1C.
Positive Control 1:
Cat. No. 1464 - Rat Liver Tissue Lysate
Purification:
JMJD1B Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
JMJD1B Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
JMJD1B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KDM3B
Additional Names:
JMJD1B Antibody: 5qNCA, NET22, C5orf7, JMJD1B, JHDM2B, KIAA1082, Lysine-specific demethylase 3B, JmjC domain-containing histone demethylation protein 2B
Protein Accession Number:
EAW62141
Protein Gi Number:
119582545
Ncbi Gene Id Number:
51780
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
JMJD1B Antibody: The jumonji domain containing 1B protein (JMJD1B) was originally discovered as a candidate for the myeloid leukemia tumor suppressor gene. Besides CD34+ cells and acute myeloid leukemia (AML) cell lines, JMJD1B mRNA is highly expressed in liver, heart, kidney, skeletal muscle, and placenta tissues. The JMJD1B gene is localized to a region of chromosome 5q31, which is frequently deleted in myeloid leukemias and myelodysplasias and expression of JMJD1B in a del(5q) cell line results in suppression of clonogenic growth suggesting that JMJD1B may function as a tumor suppressor. In contrast, JMJD1B gene copy number and mRNA expression level was increased in several non-small cell lung cancers indicating that the role of JMJD1B in cancer formation and progression is more complex than originally postulated. At least three isoforms of JMJD1B are known to exist.
Background Reference 1:
Lai F, Godley LA, Fernald AA, et al. cDNA cloning and genomic structure of three genes localized to human chromosome band 5q31 encoding potential nuclear proteins. Genomics2000; 70:123-30.
Background Reference 2:
Hu Z, Gomes I, Horrigan SK, et al. A novel nuclear protein, 5qNCA (LOC51780) is a candidate for the myeloid leukemia tumor suppressor gene on chromosome band q31. Oncogene2001; 20:6946-54.
Background Reference 3:
Baik S-H, Jee B-K, Choi J-S, et al. DNA profiling by array comparative genomic hybridization (CGH) of peripheral blood mononuclear cells (PBMC) and tumor tissue cell in non-small cell lung cancer (NSCLC). Mol. Biol. Rep.2009; 36:1767-78.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5369

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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