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Western blot analysis of JMJD1C in human liver tissue lysate with JMJD1C antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of JMJD1C in rat liver tissue with JMJD1C antibody at 2.5 μg/mL.
Immunofluorescence of JMJD1C in rat liver tissue with JMJD1C antibody at 20 μg/mL.
Western blot analysis of JMJD1C in human liver tissue lysate with JMJD1C antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

JMJD1C Antibody

CATALOG NUMBER: 5371

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
JMJD1C antibody was raised against a 20 amino acid synthetic peptide from near the amino terminus of human JMJD1C.
The immunogen is located within amino acids 380 - 430 of JMJD1C.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
JMJD1C antibody can be used for detection of JMJD1C by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificity:
This antibody will not cross-react with JMJD1A or JMJD1B.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Purification:
JMJD1C Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
JMJD1C Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
JMJD1C antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
JMJD1C
Additional Names:
JMJD1C Antibody: TRIP8, JHDM2C, KIAA1380, TRIP8, Probable JmjC domain-containing histone demethylation protein 2C, Jumonji domain-containing protein 1C, TR-interacting protein 8
Protein Accession Number:
NP_116165
Protein Gi Number:
118600981
Ncbi Gene Id Number:
221037
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
JMJD1C Antibody: The jumonji domain containing 1C protein (JMJD1C) was initially discovered in silico, and later suggested to be a candidate gene for autism. Like the related proteins JMJD1A and JMJD1B, JMJD1C is a histone H3K9 demethylase implicated in the nuclear hormone receptor-based transcriptional regulation. JMJD1C mRNA is highly expressed in undifferentiated embryonic stem (ES) cells as well as pancreatic islet, diffuse-type gastric cancer, and other tissues and tumors. The JMJD1C gene promoter contain bHLH-, AP-1-, and POU5F1-binding sites, and as preferential expression of POU5F1 has been reported in ES cells, pancreatic islet, and diffuse-type gastric cancer, it has been suggested that POU5F1-mediated expression of JMJD1C reactivates previously silenced genes in ES cells and diffuse-type gastric cancer. At least three isoforms of JMJD1C are known to exist.
Background Reference 1:
Katoh M and Katoh M. Identification of TRIP8 gene in silico. Int. J. Mol. Med.2003; 12:817-21.
Background Reference 2:
Castermans D, Vermeesch JR, Fryns JP, et al. Identification and characterization of the TRIP8 and REEP3 genes on chromosome 10q21.3 as novel candidate genes for autism. Eur. J. Hum. Genet.2007; 15:422-31.
Background Reference 3:
Katoh M and Katoh M. Comparative integromics on JMJD1C gene encoding histone demethylase: Conserved POU5F1 binding site elucidating mechanism of JMJD1C expression in undifferentiated ES cells and diffuse-type gastric cancer. Int. J. Oncology2007; 31:219-23.
Background Reference 4:
Katoh Y and Katoh M. Conserved POU-binding site linked to SP1-binding site within FZD5 promoter: transcriptional mechanism of FZD5 in undifferentiated human ES cells, fetal liver/spleen, adult colon, pancreatic islet, and diffuse-type gastric cancer. Int. J. Oncol.2007; 30:751-5.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5371

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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