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Western blot analysis of SKA2 in 3T3 cell lysate with SKA2 antibody at (A) 0.5 and (B) 1 μg/mL.
Western blot analysis of SKA2 in 3T3 cell lysate with SKA2 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

SKA2 Antibody

CATALOG NUMBER: 5403

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (93%), Rat: (80%)
Immunogen:
SKA2 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human SKA2.
The immunogen is located within the first 50 amino acids of SKA2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
SKA2 antibody can be used for detection of SKA2 by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Purification:
SKA2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SKA2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SKA2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SKA2
Additional Names:
SKA2 Antibody: FAM33A, FAM33A, Spindle and kinetochore-associated protein 2, Protein FAM33A
Protein Accession Number:
NP_872426
Protein Gi Number:
32699054
Ncbi Gene Id Number:
348235
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SKA2 Antibody: Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Depletion of either SKA1 or SKA2 by siRNA results in the loss of both proteins from the KT, but does not impact overall KT structure. Cells depleted of the SKA complex undergo a prolonged checkpoint-dependent delay in a metaphase-like state, indicating the importance of the SKA complex in the maintenance of the metaphase plate and spindle checkpoint silencing. SKA2 has also been shown to interact with glucocorticoid receptors and to be involved in glucocorticoid signaling and cell proliferation.
Background Reference 1:
Cleveland DW, Mao Y, and Sullivan KF. Centromeres and kinetochores: from epigenetics to mitotic checkpoint signaling. Cell2003; 112:407-21.
Background Reference 2:
Hanisch A, Sillje HHW, and Nigg EA. Timely anaphase onset requires a novel spindle and kinetochore complex comprising Ska1 and Ska EMBO J.2006; 25:5504-15.
Background Reference 3:
Gaitanos TN, Santamaria A, Jeyaprakash AA, et al. Stable kinetochore-microtubule interactions depend on the Ska complex and its new component Ska3/C13Orf EMBO J.2009; 28:1442-52.
Background Reference 4:
Rice L, Water CE, Eccles J, et al. Identification and functional analysis of SKA2 interaction with the glucocorticoid receptor. J. Endocrinol.2008; 198:499-509.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5403

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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