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Western blot analysis of PLEKHM3 in mouse skeletal muscle tissue lysate with PLEKHM3 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of PLEKHM3 in human brain tissue with PLEKHM3 antibody at 5 μg/mL.
Immunofluorescence of PLKHM3 in human brain tissue with PLKHM3 antibody at 20 μg/mL.
Western blot analysis of PLEKHM3 in mouse skeletal muscle tissue lysate with PLEKHM3 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

PLEKHM3 Antibody

CATALOG NUMBER: 5487

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PLEKHM3 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human PLEKHM3.
The immunogen is located within the last 50 amino acids of PLEKHM3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
PLEKHM3 antibody can be used for detection of PLEKHM3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1407 - Mouse Skeletal Muscle Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
PLEKHM3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PLEKHM3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PLEKHM3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PLEKHM3
Additional Names:
PLEKHM3 Antibody: DAPR, PLEKHM1L, Pleckstrin homology domain-containing family M member 3, PH domain-containing family M member 3
Protein Accession Number:
Q6ZWE6
Protein Gi Number:
172046173
Ncbi Gene Id Number:
389072
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PLEKHM3 Antibody: PLEKHM3, also known as DAPR, is a member of the M family of Pleckstrin homology domain-containing proteins. PLEKHM3 was initially identified through chromatin immunoprecipitation and CpG microarray analysis examining proteins regulated by myocyte-enhancing factor 2. In C2C12 myoblast cells, PLEKHM3 binds to the PI3K signaling member protein kinase B in the cytosol prior to differentiation into myotubes. Following the initiation of differentiation, PLEKHM3 was also found in membrane fractions. Knockdown of PLEKHM3 expression by RNAi resulted in the inhibition of myotube formation, suggesting that PLEKHM3 is a key component required by myoblasts for orchestrating their differentiation during myogenesis.
Background Reference 1:
Virtanen C, Paris J, and Takahashi M. Identification and characterization of a novel gene, dapr, involved in skeletal muscle differentiation and protein kinase B signaling. J. Biol. Chem.2009; 284:1636-43.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5487

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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