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Western blot analysis of TSPAN9 in EL4 cell lysate with TSPAN9 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of TSPAN9 in human spleen tissue with TSPAN9 antibody at 2.5 μg/mL.
Immunofluorescence of TSPAN9 in human spleen tissue with TSPAN9 antibody at 20 μg/mL.
Western blot analysis of TSPAN9 in EL4 cell lysate with TSPAN9 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

TSPAN9 Antibody

CATALOG NUMBER: 5559

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Sheep: (100%), Pig: (93%)
Immunogen:
TSPAN9 antibody was raised against a 15 amino acid synthetic peptide from near the center of human TSPAN9.
The immunogen is located within amino acids 90 - 140 of TSPAN9.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TSPAN9 antibody can be used for detection of TSPAN9 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1287 - EL4 Cell Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Purification:
TSPAN9 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TSPAN9 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TSPAN9 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TSPAN9
Additional Names:
TSPAN9 Antibody: NET5, NET-5, PP1057, NET5, Tetraspanin-9, Tetraspan NET-5, Tspan-9
Protein Accession Number:
NP_006666
Protein Gi Number:
5729941
Ncbi Gene Id Number:
10867
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TSPAN9 Antibody: The tetraspan family protein members are characterized by four predicted transmembrane domains and are thought to be involved in physiological processes such as tissue differentiation, immunological responses, and sperm-egg fusion. TSPAN9 has recently been identified as a platelet tetraspanin and a component of tetraspanin microdomains that include the collagen receptor GPVI (glycoprotein VI) and integrin alpha6beta1 but not the von Willebrand receptor GPIbalpha or the integrins alphaIIbbeta3 or alpha2beta1, suggesting that TSPAN9 may act to regulate platelet function in concert with other tetraspanins and their associated proteins.
Background Reference 1:
Berditchevski F and Odintsova E. Tetraspanins as regulators of protein trafficking. Traffic2007; 8:89-96.
Background Reference 2:
Serru V, Dessen P, Boucheix C, et al. Sequence and expression of seven new tetraspans. Biochim. Biophys. Acta2000; 1478:159-63.
Background Reference 3:
Protty MB, Watkins NA, Colombo D, et al. Identification of Tspan9 as a novel platelet tetraspanin and the collagen receptor GPVI as a component of tetraspanin microdomains. Biochem. J.2009; 417:391-400.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5559

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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