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Western blot analysis of REEP4 in human lung tissue lysate with REEP4 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of REEP4 in human lung tissue lysate with REEP4 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

REEP4 Antibody

CATALOG NUMBER: 5575

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
REEP4 antibody was raised against a 17 amino acid synthetic peptide near the center of human REEP4.
The immunogen is located within amino acids 160 - 210 of REEP4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
REEP4 antibody can be used for detection of REEP4 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1302 - Human Lung Tissue Lysate
Purification:
REEP4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
REEP4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
REEP4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
REEP4
Additional Names:
REEP4 Antibody: PP432, C8orf20, PP432, Receptor expression-enhancing protein 4
Protein Accession Number:
EAW63714
Protein Gi Number:
119584118
Ncbi Gene Id Number:
80346
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
REEP4 Antibody: Mammalian odorant receptors require accessory proteins such as RTP1 and RTP2 for functional cell surface expression. Receptor expression-enhancing protein (REEP) family members are transmembrane proteins which interact with odorant receptor proteins and may enhance the odorant receptor responses to odorants. Recently studies have shown other chemosensory receptors such as bitter taste receptors are also influenced by RTP and REEP family members. In studies in Xenopus RNAi to reduce REEP4 levels, embryos showed a slightly kinked body axis and were paralyzed. Further analysis revealed downregulated levels of several neural and muscle markers, suggesting the REEP4 may play a role in the maintenance of both the nervous system and musculature.
Background Reference 1:
Saito H, Kubota M, Roberts RW, et al. RTP family members induce functional expression of mammalian odorant receptors. Cell2004; 119:679-91.
Background Reference 2:
Behrens M, Bartelt J, Reichling et al. Members of RTP and REEP gene families influence functional bitter taste receptor expression. J. Biol. Chem.2006; 281:20650-9.
Background Reference 3:
Argasinska J, Rana AA, Gilchrist MJ, et al. Loss of REEP4 causes paralysis of the Xenopus embryo. Int. J. Dev. Biol.2009; 52:37-43.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5575

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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