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Western blot analysis of CHORDC1 in 293 cell lysate with CHORDC1 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of CHORDC1 in 293 cells with CHORDC1 antibody at 2.5 μg/mL.
Immunofluorescence of CHORDC1 in 293 cells with CHORDC1 antibody at 20 μg/mL.
Western blot analysis of CHORDC1 in 293 cell lysate with CHORDC1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

CHORDC1 Antibody

CATALOG NUMBER: 5587

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (100%), Bovine: (100%), Chicken: (86%)
Immunogen:
CHORDC1 antibody was raised against a 21 amino acid synthetic peptide near the amino terminus of human CHORDC1.
The immunogen is located within amino acids 50 - 100 of CHORDC1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
CHORDC1 antibody can be used for detection of CHORDC1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2:
Cat. No. 17-010 - HEK293 Cell Slide
Purification:
CHORDC1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CHORDC1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CHORDC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CHORDC1
Additional Names:
CHORDC1 Antibody: CHP1, CHP1, Cysteine and histidine-rich domain-containing protein 1, CHORD domain-containing protein 1, CHORD-containing protein 1
Protein Accession Number:
NP_036256
Protein Gi Number:
221316566
Ncbi Gene Id Number:
26973
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CHORDC1 Antibody: The cysteine and histidine-rich domain (CHORD)-containing protein (CHORDC1) is a member of a highly conserved protein family that contains the plant protein RAR1 and the mammalian protein melusin. In mammals, CHORDC1 is an ADP-dependent HSP90-interacting protein, and this interaction is dependent on the ability of HSP90 to bind nucleotides. Recent experiments indicate that CHORDC1 mRNA is diurnally regulated in mouse hypothalamus, and that this regulation alters during development, suggesting that CHORDC1 may play a role in circadian mechanisms in the mammalian brain.
Background Reference 1:
Shirasu K, Lahaye T, Tan MW, et al. A novel class of eukaryotic zinc-binding proteins is required for disease resistance signaling in barley and development in C. elegans. Cell1999; 99:355-66.
Background Reference 2:
Brancaccio M, Menini N, Bongianni D, et al. Chp-1 and melusin, two CHORD containing proteins in vertebrates. FEBS Lett.2003; 551:47-52.
Background Reference 3:
Wu J, Luo S, Jiang H, et al. Mammalian CHORD-containing protein 1 is a novel heat shock protein 90-interacting protein. FEBS Lett.2005; 579:421-6.
Background Reference 4:
Gano JJ and Simon JA. A proteomic investigation of ligand-dependent HSP90 complexes reveals CHORDC1 as a novel ADP-dependent HSP90-interacting protein. Mol. Cell Proteomics2010; 9:255-70.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5587

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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