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Western blot analysis of RSRC1 in HepG2 cell lysate with RSRC1 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of RSRC1 in rat liver tissue with RSRC1 antibody at 5 μg/mL.
Immunofluorescence of RSRC1 in rat liver tissue with RSRC1 antibody at 20 μg/mL.
Western blot analysis of RSRC1 in HepG2 cell lysate with RSRC1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

RSRC1 Antibody

CATALOG NUMBER: 5607

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
RSRC1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human RSRC1.
The immunogen is located within the last 50 amino acids of RSRC1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
RSRC1 antibody can be used for detection of RSRC1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Purification:
RSRC1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RSRC1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RSRC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RSRC1
Additional Names:
RSRC1 Antibody: BM-011, SFRS21, SRrp53, SRRP53, BM-011SRrp53
Protein Accession Number:
NP_057709
Protein Gi Number:
38488727
Ncbi Gene Id Number:
51319
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RSRC1 Antibody: The arginine/serine-rich coiled-coil 1 protein (RSRC1) was initially identified as a non-snRNP splicing factor that has a role in both the constitutive and alternative splicing of pre-mRNA molecules. In cellular nuclear extracts immunodepleted of RSRC1, the second step of pre-mRNA splicing is blocked; recombinant RSRC1 is able to restore splicing activity. RSRC1 has also been reported to be associated with schizophrenia as well as a unique marker of bone marrow-derived stem cells that also express the cdc34 marker.
Background Reference 1:
Cazalla D, Newton K, and Caceres JF. A noel SR-related protein is required for the second step of pre-mRNA splicing. Mol. Cell. Biol.2005; 25:2969-80.
Background Reference 2:
Potkin SG, Turner JA, Fallon JA, et al. Gene discovery through imaging genetics: identification of two novel genes associated with schizophrenia. Mol. Psych.2009; 14:416-28.
Background Reference 3:
Zhang QH, Ye M, Wu XY, et al. Cloning and functional analysis of cDNAs with open reading frames for 300 previously unidentified genes expressed in CD34+ hematopoietic stem/progenitor cells. Genome Res./i> 2008; 18:640-3.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5607

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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