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<strong>WB Validation in 293 Cells</strong><br> Loading: 10 µg of cell lysate Antibodies: LZTR2 5623-1001, 0.5 ug/ml, 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 10 secLane 1: In the absence of peptide blockingLane 2: In the presence of peptide blocking
Immunohistochemistry of LZTR2 in mouse kidney tissue with LZTR2 antibody at 5 μg/mL.
Immunofluorescence of LZTR2 in mouse kidney tissue with LZTR2 antibody at 20 μg/mL.
WB Validation in 293 Cells
Loading: 10 µg of cell lysate Antibodies: LZTR2 5623-1001, 0.5 ug/ml, 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 10 secLane 1: In the absence of peptide blockingLane 2: In the presence of peptide blocking
Trial Size

LZTR2 Antibody

CATALOG NUMBER: 5623

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Rabbit: (94%)
Immunogen:
LZTR2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human LZTR2.
The immunogen is located within the last 50 amino acids of LZTR2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
LZTR2 antibody can be used for detection of LZTR2 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
LZTR2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
LZTR2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
LZTR2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SEC16B
Additional Names:
LZTR2 Antibody: RGPR, LZTR2, SEC16S, PGPR-p117, KIAA1928, RGPR, Protein transport protein Sec16B, Leucine zipper transcription regulator 2, RGPR-p117
Protein Accession Number:
NP_149118
Protein Gi Number:
116235480
Ncbi Gene Id Number:
89866
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LZTR2 Antibody: LZTR2, also known as RGPR-p117, is a member of the BTB-kelch superfamily and was initially described as a nuclear factor I (NFI) binding protein and transcriptional regulator of the regucalcin gene. LZTR2 is cytoplasmically localized but is thought to translocate to the nucleus, a process mediated by protein kinase C signaling following hormonal stimulation. Recent evidence has suggested that there is a strong correlation of single nucleotide polymorphisms of LZTR2 with obesity in the Japanese population similar to that seen with the TMEM18 gene and the GNPDA2, BDNF, FAIM2, and MC4R genes with obesity in Caucasian populations, suggesting LZTR2 may play a role in metabolism and obesity risk.
Background Reference 1:
Misawa H and Yamaguchi M. Molecular cloning and sequencing of the cDNA coding for a novel regucalcin gene promoter region-related protein in rat, mouse and human liver. Int. J. Mol. Med.2001; 8:513-20.
Background Reference 2:
Yamaguchi M. Novel protein RGPR-p117: its role as the regucalcin gene transcription factor. Mol. Cell. Biochem.2009; 327:53-63.
Background Reference 3:
Hotta K, Nakamura M, Nakamura T, et al. Association between obesity and polymorphisms in SEC16B, TMEM18, GNPDA2, BDNF, FAIM2 and MC4R in a Japanese population. J. Hum. Genet.2009; 54:727-31.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5623

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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