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Western blot analysis of DCLK3 in K562 cell lysate with DCLK3 antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of DLCK3 in human brain tissue with DLCK3 antibody at 20 μg/mL.
Immunohistochemistry of DCLK3 in mouse brain tissue with DCLK3 Antibody at 5 μg/mL.
Immunofluorescence of DCLK3 in mouse brain tissue with DCLK3 Antibody at 20 μg/mL. <br><br>Green: DCLK3 antibody (5637) <br> Red: Phylloidin staining<br> Blue: DAPI staining
Western blot analysis of DCLK3 in K562 cell lysate with DCLK3 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

DCLK3 Antibody

CATALOG NUMBER: 5637

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
DCLK3 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human DCLK3.
The immunogen is located within the last 50 amino acids of DCLK3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
DCLK3 antibody can be used for detection of DCLK3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
DCLK3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
DCLK3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
DCLK3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DCLK3
Additional Names:
DCLK3 Antibody: CLR, DCK3, DCDC3C, DCAMKL3, KIAA1765, Doublecortin domain-containing protein 3C
Protein Accession Number:
NP_208382
Protein Gi Number:
149589021
Ncbi Gene Id Number:
85443
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DCLK3 Antibody: DCLK3 is one of three doublecortin-like kinases similar to the Ca2+/calmodulin-dependent protein kinase (CaMK) family. DCLK3 mRNA, like that of the homologous DCLK1 and DCLK3, is highly expressed in adult brain, but only DCLK3 transcripts are present in liver and kidney, suggesting that DCLK3 may play other roles than in cortical development. The DCLK proteins are homologous to Doublecortin (DCX), a protein that is mutated in X-linked human lissencephaly. In mouse models where the DCX gene has been disrupted, DCLK1 expression increases slightly and appears to compensate for the loss of DCX, as mice mutant for both DCX and DCLK1 show a severe phenotype including perinatal lethality, disorganized neocortical layering, and profound hippocampal cytoarchitectural disorganization.
Background Reference 1:
Ohmae S, Takemoto-Kimura S, Okamura M, et al. Molecular identification and characterization of a family of kinases with homology to Ca2+/calmodulin-dependent protein kinases I/IV. J. Biol. Chem.2006; 281:20427-39.
Background Reference 2:
Sossey-Alaoui K and Srivastava AK. DCAMKL1, a brain specific transmembrane protein on 13q12.3 that is similar to doublecortin (DCX), Genomics1999; 56:121-6.
Background Reference 3:
Tuy FPD, Saillour Y, Kappeler C, et al. Alternative transcripts of Dlck1 and Dlck2 and their expression in doublecortin knockout mice. Dev. Neurosci.2008; 30:171-86.
Background Reference 4:
Reiner O and Coquelle FM. Missense mutations resulting in type 1 lissencephaly. Cell Mol. Life Sci.2005; 62:425-34.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5637

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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