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Western blot analysis of ZC3H12C in rat brain tissue lysate with ZC3H12C antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of ZC3H12C in human brain tissue with ZC3H12C antibody at 5 μg/mL.
Immunofluorescence of ZC3H12C in human brain tissue with ZC3H12C antibody at 20 μg/mL.
Western blot analysis of ZC3H12C in rat brain tissue lysate with ZC3H12C antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

ZC3H12C Antibody

CATALOG NUMBER: 5677

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
ZC3H12C antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human ZC3H12C.
The immunogen is located within the first 50 amino acids of ZC3H12C.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ZC3H12C antibody can be used for detection of ZC3H12C by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
ZC3H12C Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZC3H12C Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZC3H12C antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZC3H12C
Additional Names:
ZC3H12C Antibody: MCPIP3, KIAA1726, MCPIP3, Probable ribonuclease ZC3H12C, MCP-induced protein 3
Protein Accession Number:
NP_203748
Protein Gi Number:
148886668
Ncbi Gene Id Number:
85463
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZC3H12C Antibody: ZC3H12C, also known as MCPIP3, is a member of a family of novel CCCH-zinc finger proteins that includes ZC3H12A, a protein that is thought to be involved in macrophage activation, host immunity and inflammatory diseases. Similar to ZC3H12A, ZC3H12C expression in macrophages is highly increased after treatment with lipopolysaccharide (LPS), suggesting it also may play a role in host immunity and inflammatory response.
Background Reference 1:
Zhou L, Azfer A, Niu J, et al. Monocyte chemoattractant protein-1 induces a novel transcription factor that causes cardiac myocyte apoptosis and ventricular dysfunction. Circ. Res.2006; 98:1177-85.
Background Reference 2:
Liang J, Wang J, Azfer A, et al. A novel CCCH-zinc finger protein family regulates proinflammatory activation of macrophages. J. Biol. Chem.2008; 283:6337-46.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5677

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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