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<strong>Figure 1 WB Validation in Human Cell Lines</strong><br>Loading: 15 μg of lysate. Antibodies: CCDC69 5691, 1 μg/mL , 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 2 Western Blot Validation in Mouse Pancreas Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CCDC69 5691, 1 μg/mL, 1h incubation at RT in 8% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 3 Western Blot Validation in Rat Pancreas Lysate</strong><br>Loading: 15 μg of lysates per lane. Antibodies: CCDC69 5691, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
Immunohistochemistry of CCDC69 in human lung tissue with CCDC69 antibody at 5 μg/mL.
Immunofluorescence of CCDC69 in human lung tissue with CCDC69 antibody at 20 μg/mL.
Figure 1 WB Validation in Human Cell Lines
Loading: 15 μg of lysate. Antibodies: CCDC69 5691, 1 μg/mL , 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
Trial Size

CCDC69 Antibody

CATALOG NUMBER: 5691

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (78%)
Immunogen:
Anti-CCDC69 antibody (5691) was raised against an 18 amino acid synthetic peptide from near the center of human CCDC69.
The immunogen is located within amino acids 150 - 200 of CCDC69.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
WB: 1-2 μg/mL; IF: 5-20 μg/mL; IHC: 1-5 μg/mL.
Antibody validated: Western Blot in human, mouse and ratsamples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1402 - Mouse Lung Tissue Lysate
Positive Control 2:
Cat. No. 10-101 - Human Lung Tissue Slide
Predicted Molecular Weight:
Predicted: 35kD
Observed: 35 kD
Isoforms:
Human CCDC69 has 1 isoform (296aa, 34.8kD). Mouse CCDC69 has 2 isoforms, isoform 1 (202aa, 23.3kD) and isoform 2 (176aa, 20.2 kD). Rat CCDC69 has 1 isoform (202aa, 23.5kD). 5691 can detect CCDC69 in human, mouse, and rat.
Purification:
CCDC69 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgY
physical-state:
Liquid
Buffer:
CCDC69 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CCDC69 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CCDC69
Additional Names:
CCDC69 Antibody: Coiled-coil domain-containing protein 69
Protein Accession Number:
NP_056436
Protein Gi Number:
45935391
Ncbi Gene Id Number:
26112
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CCDC69 Antibody: The coiled-coil domain is a common protein motif that is often involved in protein oligomerization and is found in proteins such as transcription factors and intermediate filaments. The CCDC47 gene maps to chromosome 5 at 5q33.1. Little is known about this protein.
Background Reference 1:
Steinmetz MO, Jelesarov I, Matousek WM, et al. Molecular basis of coiled-coil formation. Proc. Natl. Acad. Sci. USA2007; 104:7062-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5691

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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