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Western blot analysis of PIAS2 in rat brain tissue lysate with PIAS2 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of PIAS2 in rat brain tissue with PIAS2 antibody at 5 μg/mL.
Immunofluorescence of PIAS2 in rat brain tissue with PIAS2 antibody at 20 μg/mL.
Western blot analysis of PIAS2 in rat brain tissue lysate with PIAS2 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

PIAS2 Antibody

CATALOG NUMBER: 5743

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PIAS2 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human PIAS2.
The immunogen is located within amino acids 110 - 160 of PIAS2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
PIAS2 antibody can be used for detection of PIAS2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Purification:
PIAS2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PIAS2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PIAS2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PIAS2
Additional Names:
PIAS2 Antibody: DIP, MIZ, MIZ1, SIZ2, ARIP3, PIASX, ZMIZ4, PIASX-BETA, PIASX-ALPHA, E3 SUMO-protein ligase PIAS2, Androgen receptor-interacting protein 3
Protein Accession Number:
EAX01485
Protein Gi Number:
119621890
Ncbi Gene Id Number:
9063
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PIAS2 Antibody: The PIAS (protein inhibitor of activated STAT) proteins play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS2. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS2 binds specifically to STAT4 following IL-12 stimulation and inhibits STAT4-mediated gene activation in human T cells. PIAS2 is a potent transcriptional activator of Bcl-2, but together with Bcl-6 can suppress the expression of Bcl-2.
Background Reference 1:
Shuai K and Liu B. Regulation of gene-activation pathways by PIAS proteins in the immune system. Nat. Rev. Immunol.2005; 5:593-605.
Background Reference 2:
Arora T, Liu B, He H, et al. PIASx is a transcriptional co-repressor of signal transducer and activator of transcription 4. J. Biol. Chem.2003; 278:21327-30.
Background Reference 3:
Patel JH and McMahon SB. BCL2 is a downstream effector of MIZ-1 essential for blocking c-MYC-induced apoptosis. J. Biol. Chem.2007; 282:5-13.
Background Reference 4:
Saito M, Novak U, Piovan E, et al. BCL6 suppression of BCL2 via Miz1 and its disruption in diffuse large B cell lymphoma. Proc. Natl. Acad. Sci. USA2009; 106:11294-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5743

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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