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Western blot analysis of APC11 in HeLa cell lysate with APC11 antibody at 1 μg/mL.
Immunofluorescence of APC11 in 3T3 cells with APC11 antibody at 20 μg/mL.
Western blot analysis of APC11 in HeLa cell lysate with APC11 antibody at 1 μg/mL.
Trial Size

APC11 Antibody

CATALOG NUMBER: 5737

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
APC11 antibody was raised against an 18 amino acid synthetic peptide near the center of human APC11.
The immunogen is located within amino acids 70 - 120 of APC11.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
APC11 antibody can be used for detection of APC11 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-201 - 3T3/BALB Cell Slide
Predicted Molecular Weight:
Predicted: 22 kDa
Observed: 22 kDa
Purification:
APC11 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
APC11 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
APC11 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ANAPC11
Additional Names:
APC11 Antibody: APC11, Apc11p, HSPC214, Anaphase-promoting complex subunit 11, Cyclosome subunit 11, APC11
Protein Accession Number:
NP_001002244
Protein Gi Number:
50409810
Ncbi Gene Id Number:
51529
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
APC11 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC11 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. APC11, together with APC2 and Ubc4, form the minimal APC ubiquitin ligase module.
Background Reference 1:
JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56.
Background Reference 2:
Jorgensen PM, Graslund S, Betz R, et al. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene2001; 262:51-9.
Background Reference 3:
Tan Z, Li B, Bharadwaj R, et al. APC Cullin protein and APC RING protein comprise the minimal ubiquitin ligase module of the anaphase-promoting complex. Mol. Biol. Cell2001; 12:3839-51.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5737

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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