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Western blot analysis of FOXO3 in A-20 cell lysate with FOXO3 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunocytochemistry of FOXO3 in A-20 cells with FOXO3 antibody at 4 μg/mL.
Western blot analysis of FOXO3 in A-20 cell lysate with FOXO3 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

FOXO3 Antibody

CATALOG NUMBER: 5813

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
FOXO3 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human FOXO3.
The immunogen is located within amino acids 40 - 90 of FOXO3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
FOXO3 antibody can be used for detection of FOXO3 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Positive Control 2:
Cat. No. 17-208 - A-20 Cell Slide
Purification:
FOXO3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
FOXO3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
FOXO3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FOXO3
Additional Names:
FOXO3 Antibody: FOXO2, AF6q21, FKHRL1, FOXO3A, FKHRL1P2, Forkhead box protein O3, AF6q21 protein
Protein Accession Number:
NP_963853
Protein Gi Number:
42519916
Ncbi Gene Id Number:
2309
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FOXO3 Antibody: FOXO3 is a ubiquitously expressed 75 kDa protein member of a subfamily of the forkhead homeotic gene family of transcription factors and shuttles between the cytoplasm and nucleus. FOXO transcription factors are key players of cell fate decisions, metabolism, stress resistance, tumor suppression and are regulated by growth factors, oxidative stress or nutrient deprivation. FOXO3 is involved with mTOR in the regulation of autophagy in skeletal muscle, and activates protein degradation in atrophying muscle cells. FOXO3 has also been implicated in several neurodegenerative disorders including aging, neuromuscular disease, systemic lupus erythmatosus, stroke and diabetic complications.
Background Reference 1:
Anderson MJ, Viars CS, Czekay S, et al. Cloning and characterization of three human forkhead genes that comprise an FKHR-like gene subfamily. Genomics1998; 47:187-99.
Background Reference 2:
Greer EL and Brunet A. FOXO transcription factors at the interface between longevity and tumor suppression. Oncogene2005; 24:7410-25.
Background Reference 3:
Mammucari C, Schiaffino S, and Sandri M. Downstream of Akt: FoxO3 and mTOR in the regulation of autophagy in skeletal muscle. Autophagy2008; 4:524-6.
Background Reference 4:
Zhao J, Brault JJ, Schild A, et al. FoxO3 coordinately activates protein degradation by the autophagic/lysosomal and proteasomal pathways in atrophying muscle cells. Cell Metab.2007; 6:472-83.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5813

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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