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Western blot analysis of IDH2 in human heart tissue lysate with IDH2 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of IDH2 in mouse heart tissue with IDH2 antibody at 5 μg/mL.
Immunofluorescence of IDH2 in mouse heart tissue with IDH2 antibody at 20 μg/mL.
Western blot analysis of IDH2 in human heart tissue lysate with IDH2 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

IDH2 Antibody

CATALOG NUMBER: 5821

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (88%)
Immunogen:
IDH2 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human IDH2.
The immunogen is located within the first 50 amino acids of IDH2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
IDH2 antibody can be used for detection of IDH2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Purification:
IDH2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IDH2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IDH2 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IDH2
Additional Names:
IDH2 Antibody: IDH, IDP, IDHM, IDPM, ICD-M, D2HGA2, mNADP-IDHICD-M, IDH
Protein Accession Number:
NP_002159
Protein Gi Number:
28178832
Ncbi Gene Id Number:
3418
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IDH2 Antibody: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Two NADP(+)-dependent isocitrate dehydrogenases have been found as homodimer: IDH1 is predominantly cytosolic and peroxisomal and IDH2 is mitochondrial. Both IDH1 and IDH2 play significant roles in the tricarboxylic acid cycle (TCA), and defects in IDH1 as well as IDH2 have been implicated in the development of glioma as well as other malignancies.
Background Reference 1:
Geisbrecht BV and Gould SJ. The human PICD gene encodes a cytoplasmic and peroxisomal NADP(+)-dependent isocitrate dehydrogenase. J. Biol. Chem.1999; 274:30527-33.
Background Reference 2:
Przybyla-Zawislak B, Gadde DM, Ducharme K, et al. Genetic and biochemical interactions involving tricarboxylic acid cycle (TCA) function using a collection of mutants defective in all TCA cycle genes. Genetics1999; 152:153-66.
Background Reference 3:
Dang L, White DW, and Gross S. Cancer-associated IDH1 mutations produce 2-hydroxyglutarate. Nature2009; 462:739-44.
Background Reference 4:
Tan H, Parsons DW, Jin G, et al. IDH1 and IDH2 mutations in gliomas. N. Engl. J. Med.2009; 360:765-73.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5821

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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