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Western blot analysis of ZEB2 in EL4 cell lysate with ZEB2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of ZEB2 in Jurkat cells with ZEB2 antibody at 20 μg/mL.
Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 μg/ml.<br><br>Red: ZEB2 Antibody (5827) <br> Blue: DAPI staining
Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 μg/mL.
Western blot analysis of ZEB2 in EL4 cell lysate with ZEB2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

ZEB2 Antibody

CATALOG NUMBER: 5827

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
ZEB2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human ZEB2.
The immunogen is located within the last 50 amino acids of ZEB2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
ZEB2 antibody can be used for detection of ZEB2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
ZEB2 antibody is predicted to not cross-react with ZEB1.
Positive Control 1:
Cat. No. 1287 - EL4 Cell Lysate
Positive Control 2:
Cat. No. 17-005 - Jurkat Cell Slide
Predicted Molecular Weight:
Predicted: 134 kDa
Observed: 135 kDa
Purification:
ZEB2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZEB2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZEB2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZEB2
Additional Names:
ZEB2 Antibody: SIP1, SIP-1, ZFHX1B, HSPC082, SMADIP1, KIAA0569, SIP1, ZFX1B, HRIHFB2411, Zinc finger E-box-binding homeobox 2, Smad-interacting protein 1
Protein Accession Number:
AAI27103
Protein Gi Number:
117558786
Ncbi Gene Id Number:
9839
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZEB2 Antibody: ZEB2, initially identified as Smad interacting-protein 1, is normally located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Like the homologous ZEB1, ZEB2 inhibits the transcription of the E-cadherin gene and induces epithelial-mesenchymal transition, a genetic program controlling cell migration during embryonic development and wound healing, in vitro. ZEB2 can also protect cells from DNA damage-induced apoptosis, suggesting that its expression may contribute to tumor progression. Recent evidence has shown that ZEB2 is often observed in the cytoplasm in numerous cancer tissues, indicating that its localization may be regulated in normal and tumor tissues. Mutations in this gene are also associated with Hirschsprung disease/Mowat-Wilson syndrome.
Background Reference 1:
Comjin J, Berx G, Vermassen P, et al. The two-handed E box binding zinc finger protein SIP1 down regulates E-cadherin and induces invasion. Mol. Cell2001; 7:1267-78.
Background Reference 2:
Sayan AE, Griffiths TR, Pal R, et al. SIP1 protein protects cells from DNA damage-induced apoptosis and has independent prognostic value in bladder cancer. Proc. Natl. Acad. Sci. USA2009; 106:14884-9.
Background Reference 3:
Oztas E, Avci ME, Ozcan A, et al. Novel monoclonal antibodies detect Smad-interacting protein 1 (SIP1) in the cytoplasm of human cells from multiple tumor tissue arrays. Exp. Mol. Pathol.2010; epub
Background Reference 4:
Wakamatsu N, Yamada Y, Yamada K, et al. Mutations in SIP1, encoding Smad interacting protein-1, cause a form of Hirschsprung disease. Nat. Genet.2001; 27:369-70.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5827

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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