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Western blot analysis of FREM1 in K562 cell lysate with FREM1 antibody at (A) 0.5 and (B) 1μg/mL.
Immunocytochemistry of FREM1 in K562 cells with FREM1 antibody at 20 μg/mL.
Immunofluorescence of FREM1 in K562 cells with FREM1 antibody at 20 μg/mL.
Western blot analysis of FREM1 in K562 cell lysate with FREM1 antibody at (A) 0.5 and (B) 1μg/mL.
Trial Size

FREM1 Antibody

CATALOG NUMBER: 5829

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
FREM1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human FREM1.
The immunogen is located within the last 50 amino acids of FREM1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
FREM1 antibody can be used for detection of FREM1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 2:
Cat. No. 17-004 - K-562 Cell Slide
Purification:
FREM1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
FREM1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
FREM1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FREM1
Additional Names:
FREM1 Antibody: BNAR, MOTA, TILRR, TRIGNO2, C9orf143, C9orf145, C9orf154, FRAS1-related extracellular matrix protein 1, Protein QBRICK
Protein Accession Number:
NP_659403
Protein Gi Number:
122056683
Ncbi Gene Id Number:
158326
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FREM1 Antibody: FREM1 is a member of the FRAS1-related extracellular matrix protein family and is thought to play a role in craniofacial and renal development. FREM1 functions as an extracellular matrix protein that is essential for epidermal adhesion during embryogenesis and may also participate in epidermal differentiation. It is recognized by cells in the embryonic skin and hair follicles through different members of the integrin family. Deficiency in the Fras1/Frem genes gives rise to the bleb phenotype, which is equivalent to the human hereditary disorder Fraser syndrome.
Background Reference 1:
Smyth I, Du X, Taylor MS, et al. The extracellular matrix gene FREM1 is essential for the normal adhesion of the embryonic epidermis. Proc. Natl. Acad. Sci. USA2004; 101:13560-5.
Background Reference 2:
Kiyozumi D, Osada A, Sugimoto N, et al. Identification of a novel cell-adhesive protein spatiotemporally expressed in the basement membrane of mouse developing hair follicle. Exp. Cell Res.2005; 306:9-23.
Background Reference 3:
Petrou P, Makrygiannis AK, Chalepakis G, et al. The FRAS1/FREM family of extracellular matrix proteins: structure, function, and association with Fraser syndrome and the mouse bleb phenotype. Connect. Tissue Res.2008; 49:277-82.
Background Reference 4:
Zhang X, Shephard F, Kim HB, et al. TILRR, a novel IL-1RI co-receptor, potentiates MyD88 recruitment to control Ras-dependent amplification of NF-kappaB J. Biol. Chem.2010; 285:7222-32.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5829

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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