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Western blot analysis of TCTN3 in HeLa cell lysate with TCTN3 antibody at (A) 1 and (B) 2μg/mL.
Immunocytochemistry of TCTN3 in HeLa cells with TCTN3 antibody at 4 μg/mL.
Immunofluorescence of TCTN3 in HeLa cells with TCTN3 antibody at 20 μg/mL.
Western blot analysis of TCTN3 in HeLa cell lysate with TCTN3 antibody at (A) 1 and (B) 2μg/mL.
Trial Size

TCTN3 Antibody

CATALOG NUMBER: 5837

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
TCTN3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human TCTN3.
The immunogen is located within amino acids 540 - 590 of TCTN3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TCTN3 antibody can be used for detection of TCTN3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1220 - SK-N-SH Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Purification:
TCTN3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TCTN3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TCTN3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TCTN3
Additional Names:
TCTN3 Antibody: OFD4, TECT3, JBTS18, C10orf61, PSEC0041, UNQ1881/PRO4324, Tectonic-3
Protein Accession Number:
NP_056446
Protein Gi Number:
221136774
Ncbi Gene Id Number:
26123
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TCTN3 Antibody: TCTN3 is a member of the Tectonic protein family, a group of evolutionarily conserved secreted and transmembrane proteins that regulate the Hedgehog (Hh)-mediated patterning of the neural tube. While the expression pattern and function of TCTN3 is not known, it is 58% similar to TCTN1. TCTN1 is expressed during embryonic development in regions that participate in Hh signaling, beginning in the gastrulation stages in the ventral node. Mice expressing mutant TCTN1 die between E13.5 and E16.5 and display holoprosencephaly, a defect associated with reduced Hh signaling, indicating the role of TCTN1 as an Hh activator. At later stages in development, TCTN1 is thought to also act as a repressor on the Hh pathway in the anterior and posterior neural tube.
Background Reference 1:
Reiter JF and Skarnes WC. Tectonic, a novel regulator of the Hedgehog pathwy required for both activation and inhibition. Genes Dev.2006; 20:22-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5837

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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