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Western blot analysis of ZC3HAV1 in HeLa cell lysate with ZC3HAV1 antibody at 1μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of ZC3HAV1 in HeLa cells with ZC3HAV1 antibody at 20 μg/mL.
Immunofluorescence of ZC3HAV1 in Hela cells with ZC3HAV1 antibody at 20 μg/mL.
Western blot analysis of ZC3HAV1 in HeLa cell lysate with ZC3HAV1 antibody at 1μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

ZC3HAV1 Antibody

CATALOG NUMBER: 5839

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
ZC3HAV1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human ZC3HAV1.
The immunogen is located within amino acids 680 - 730 of ZC3HAV1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
ZC3HAV1 antibody can be used for detection of ZC3HAV1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Purification:
ZC3HAV1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZC3HAV1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZC3HAV1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZC3HAV1
Additional Names:
ZC3HAV1 Antibody: ZAP, ZC3H2, ARTD13, PARP13, FLB6421, ZC3HDC2, PRO1677, Zinc finger CCCH-type antiviral protein 1, ADP-ribosyltransferase diphtheria toxin-like 13
Protein Accession Number:
NP_078901
Protein Gi Number:
27477138
Ncbi Gene Id Number:
56829
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZC3HAV1 Antibody: The zinc finger antiviral protein (ZC3HAV1) is a CCCH type zinc finger protein that induces an innate immunity to infections by retrovirus by preventing the accumulation of viral RNAs in the cytoplasm and recruits the RNA processing exosome to degrade target RNAs, thereby inhibiting virus replication. ZC3HAV1 is localized in the cytoplasm at steady state, but shuttles between nucleus and cytoplasm in a XPO1-dependent manner. ZAP is a direct target gene of IRF3 action in cellular antiviral responses.
Background Reference 1:
Gao G, Guo X, and Goff SP. Inhibition of retroviral RNA production by ZAP, a CCCH-Type zinc finger protein. Science2002; 297:1703-6.
Background Reference 2:
Guo X, Ma J, Sun J, et al. The zinc-finger antiviral protein recruits the RNA processing exosome to degrade the target mRNA. Proc. Natl. Acad. Sci. USA2007; 104:151-6.
Background Reference 3:
MacDonald MR, Machlin ES, Albin OR, et al. The zinc finger antiviral protein acts synergistically with an interferon-induced factor for maximal activity against alphaviruses. J. Virol. 2007; 81:13509-18.
Background Reference 4:
Wang N, Dong Q, Li J, et al. Viral induction of the zinc finger antiviral protein is IRF3-dependent but NF-kappaB-independent. J. Biol. Chem.2010; 285:6080-90.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5839

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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