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Western blot analysis of ATP11B in K562 cell tissue lysate with ATP11B antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of ATP11B in K562 cells with ATP11B antibody at 10 μg/mL.
Immunofluorescence of ATP11B in K562 cells with ATP11B antibody at 20 μg/mL.
Western blot analysis of ATP11B in K562 cell tissue lysate with ATP11B antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

ATP11B Antibody

CATALOG NUMBER: 5857

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (100%)
Immunogen:
ATP11B antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human ATP11B.
The immunogen is located within amino acids 290 - 340 of ATP11B.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
ATP11B antibody can be used for detection of ATP11B by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 2:
Cat. No. 17-004 - K-562 Cell Slide
Purification:
ATP11B Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ATP11B Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ATP11B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ATP11B
Additional Names:
ATP11B Antibody: ATPIF, ATPIR, ATPIF, KIAA0956, Probable phospholipid-transporting ATPase IF, ATPase IR
Protein Accession Number:
NP_055431
Protein Gi Number:
62632750
Ncbi Gene Id Number:
23200
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ATP11B Antibody: ATP11B is a widely expressed integral membrane ATPase and is thought to drive the transport of phospholipids across membranes. It has been suggested that ATP11B is hormonally regulated and plays a role in the subnuclear trafficking of transcription factors with RING motifs. While the exact molecule ATP11B transports is unknown, increased mRNA expression of the homologous ATP11A has been observed in cells resistant to anti-cancer drugs such as farnesyltransferase inhibitors (FTIs), suggesting that ATP11B may also play a role in cell survival under harsh conditions.
Background Reference 1:
Mansharamani M, Hewetson A, and Chilton BS. Cloning and characterization of an atypical Type IV P-type ATPase that binds to the RING motif of RUSH transcription factors. J. Biol. Chem.2001; 276:3641-9.
Background Reference 2:
Halleck MS, Lawler JF Jr, Blackshaw S, et al. Differential expression of putative transbilayer amphipath transporters. Physiol. Genomics1999; 1:139-50.
Background Reference 3:
Zhang B, Groffen J, and Heisterkamp N. Resistance to farnesyltransferase inhibitors in Bcr/Abl-positive lymphoblastic leukemia by increased expression of a novel ABC transporter homolog ATP11a. Blood2005; 106:1355-61.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5857

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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