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Western blot analysis of TRIM71 in human brain tissue lysate with TRIM71 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of TRIM71 in human brain tissue with TRIM71 antibody at 5 μg/mL.
Immunofluorescence of TRIM71 in Human Brain cells with TRIM71 antibody at 20 μg/mL.
Western blot analysis of TRIM71 in human brain tissue lysate with TRIM71 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

TRIM71 Antibody

CATALOG NUMBER: 5881

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (100%)
Immunogen:
TRIM71 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human TRIM71.
The immunogen is located within amino acids 680 - 730 of TRIM71.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TRIM71 antibody can be used for detection of TRIM71 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
TRIM71 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TRIM71 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TRIM71 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TRIM71
Additional Names:
TRIM71 Antibody: LIN41, LIN-41, LIN41, E3 ubiquitin-protein ligase TRIM71, Protein lin-41 homolog
Protein Accession Number:
NP_001034200
Protein Gi Number:
84993742
Ncbi Gene Id Number:
131405
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TRIM71 Antibody: TRIM71 is the mammalian ortholog to the C. elegans heterochronic protein Lin41, a protein that is thought to be important in postembryonic development, and is genetically and biochemically downstream of both Shh and Fgf signaling pathways. Similar to its C. elegans homolog, TRIM71 is a target of the microRNA let-7 and is likely to play a role in mammalian development. Recent experiments have indicated that TRIM71 is an E3 ubiquitin ligase and can interact with the Dicer and the Argonaute proteins Ago1, Ago2, and Ago4. Overexpression and depletion of TRIM71 led to inverse changes in Ago2 protein levels, suggesting TRIM71 can regulate Ago2 turnover. Finally, TRIM71 cooperates with the pluripotency factor Lin-28 in regulating let-7.
Background Reference 1:
Lancman JJ, Caruccio NC, Harfe BD, et al. Analysis of the regulation of lin-41 during chick and mouse limb development. Dev. Dyn.2005; 234:948-60.
Background Reference 2:
Lin YC, Hsieh LC, Kuo MW, et al. Human TRIM71 and its nematode homologue are targets of let-7 microRNA and its zebrafish orthologue is essential for development. Mol. Biol. Evol.2007; 24:2525-34.
Background Reference 3:
Rybak A, Fuchs H, Hadian K, et al. The let-7 target gene mouse lin-41 is a stem cell specific E3 ubiquitin ligase for the miRNA pathway protein Ago2. Nat. Cell Biol.2009; 11:1411-20.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5881

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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