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Western blot analysis of NOD5 in rat spleen tissue cell lysate with NOD5 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of NOD5 in rat spleen tissue with NOD5 antibody at 10 μg/mL.
Immunofluorescence of NOD5 in rat spleen tissue with NOD5 antibody at 20 μg/mL.
Western blot analysis of NOD5 in rat spleen tissue cell lysate with NOD5 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

NOD5 Antibody

CATALOG NUMBER: 5953

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
NOD5 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human NOD5.
The immunogen is located within amino acids 70 - 120 of NOD5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
NOD5 antibody can be used for detection of NOD5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1466 - Rat Spleen Tissue Lysate
Purification:
NOD5 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NOD5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NOD5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NLRX1
Additional Names:
NOD5 Antibody: NOD5, NOD9, NOD26, DLNB26, CLR11.3, NOD5, NLR family member X1, Nucleotide-binding oligomerization domain protein 26
Protein Accession Number:
NP_078894
Protein Gi Number:
25777608
Ncbi Gene Id Number:
79671
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NOD5 Antibody: NOD5, also known as NLRX1, is a member of the NOD (nucleotide-binding oligomerization domain) family, a group of proteins that are involved in innate immune defense. NOD5 localizes to the mitochondrial outer membrane and interacts with the virus-induced signaling adapter protein VISA. Unlike a subset of NOD-like receptors (NLRs) such as NOD1 and NOD2 which trigger pro-inflammatory cascades, and other NLRs that induce the caspase 1 inflammasome in response to immune challenges, NOD5 amplifies NF-κB and JNK pathways by inducing reactive oxygen species production.
Background Reference 1:
Kufer TA, Banks DJ, and Philpott DJ. Innate immune sensing of microbes by Nod proteins. Ann. NY Acad. Sci.2006; 1072:19-27.
Background Reference 2:
Moore CB, Bergstralh DT, Duncan JA, et al. NLRX1 is a regulator of mitochondrial antiviral immunity. Nature2008; 451:573-7.
Background Reference 3:
Tattoli I, Carneiro LA, Jehanno M, et al. NLRX1 is a mitochondrial NOD-like receptor that amplifies NF-kB and JNK pathways by inducing reactive oxygen species. EMBO Reports2008; 9:293-300.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5953

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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