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Western blot analysis of NOD6 in EL4 cell lysate with NOD6 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.
Immunohistochemistry of NOD6 in human testis tissue with NOD6 antibody at 10 μg/mL.
Immunofluorescence of NOD6 in human testis tissue with NOD6 antibody at 20 μg/mL.
Western blot analysis of NOD6 in EL4 cell lysate with NOD6 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.
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NOD6 Antibody

CATALOG NUMBER: 5955

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (93%)
Immunogen:
NOD6 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human NOD6.
The immunogen is located within amino acids 190 - 240 of NOD6.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
NOD6 antibody can be used for detection of NOD6 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1287 - EL4 Cell Lysate
Positive Control 2:
Cat. No. 11-701 - Human Testis Tissue Slide
Purification:
NOD6 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
NOD6 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NOD6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NLRP9
Additional Names:
NOD6 Antibody: NOD6, NALP9, PAN12, CLR19.1, NOD6, NACHT, LRR and PYD domains-containing protein 9, Nucleotide-binding oligomerization domain protein 6
Protein Accession Number:
Q7RTR0
Protein Gi Number:
74762418
Ncbi Gene Id Number:
338321
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NOD6 Antibody: NOD6, also known as NALP9, is a member of the NALP family, a group of proteins that typically contain a NACHT domain, a NACHT-associated domain (NAD), a C-terminal leucine-rich repeat (LRR) region, and an N-terminal pyrin domain (PYD) and are involved in inflammation and innate immune defense. The bovine NOD6, which has 76% homology to its human counterpart, has been suggested to be an oocyte marker gene. In adult tissues, NALP9 mRNA is expressed exclusively in ovary and testis.
Background Reference 1:
Tschopp J, Martinon F, and Burns K. NALPs: a novel protein family involved in inflammation. Nat. Rev. Mol. Cell Biol.2003; 4:95-104.
Background Reference 2:
Dalbies-Tran R, Papillier P, Pennetier S, et al. Bovine mater-like NALP9 is an oocyte marker gene. Mol. Reprod. Dev.2005; 71:414-21.
Background Reference 3:
Ponsuksili S, Brunner RM, Goldammer T, et al. Bovine NALP5, NALP8, and NALP9 genes: assignment to a QTL region and the expression in adult tissues, oocytes, and preimplantation embryos. Biol. Reprod.2006; 74:577-84.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5955

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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