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Western blot analysis of PIWI-L1 in human testis tissue lysate with PIWI-L2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of PIWI-L1 in rat liver tissue with PIWI-L1 antibody at 10 μg/mL.
Immunofluorescence of PIWI-L1 in rat liver tissue with PIWI-L1 antibody at 20 μg/mL.
Western blot analysis of PIWI-L1 in human testis tissue lysate with PIWI-L2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

PIWI-L1 Antibody

CATALOG NUMBER: 6029

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (94%)
Immunogen:
PIWI-L1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PIWI-L1.
The immunogen is located within amino acids 60 - 110 of PIWI-L1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
PIWI-L1 antibody can be used for detection of PIWI-L1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Positive Control 2:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
Predicted: 85, 90 kDa
Observed: 95 kDa
Purification:
PIWI-L1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PIWI-L1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PIWI-L1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PIWIL1
Additional Names:
PIWI-L1 Antibody: HIWI, MIWI, PIWI, CT80.1, HIWI, Piwi-like protein 1
Protein Accession Number:
AAK69348
Protein Gi Number:
14579645
Ncbi Gene Id Number:
9271
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PIWI-L1 Antibody: PIWI-L1 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that are crucial for the biogenesis and function of small non-coding RNAs and play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. PIWI-L1 is thought to play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells as removing PIWI-L1 protein from single germline stem cells significantly decreases the rate of their division. Recent studies have shown that overexpression of PIWI-L1 (as well as other PIWI-like proteins) are potential biomarkers for colon and other cancers.
Background Reference 1:
Thomson T and Lin H. The biogenesis and function PIWI proteins and piRNAs: progress and prospect. Annu. Rev. Cell Dev. Biol.2009; 25:355-76.
Background Reference 2:
Cox DN, Chao A, and Lin H. PIWI encodes a nucleoplasmic factor whose activity modulates the number and division rate of germline stem cells. Development2000; 503-14.
Background Reference 3:
Li L, Yu C, Gao H, et al. Argonaute proteins: potential biomarkers for human colon cancer. BMC Cancer2010; 10:38.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6029

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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