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Western blot analysis of SYNPO in rat brain tissue lysate with SYNPO antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of SYNPO in human brain tissue with SYNPO antibody at 2.5 μg/mL.
Immunofluorescence of SYNPO in human brain tissue with SYNPO antibody at 20 μg/mL.
Immunofluorescence of SYNPO in mouse brain tissue with SYNPO antibody at 20 μg/mL.<br><br>Red: SYNPO Antibody (6049) <br> Blue: DAPI staining
Immunohistochemistry of SYNPO in mouse brain tissue with SYNPO antibody at 5 μg/mL.
Western blot analysis of SYNPO in rat brain tissue lysate with SYNPO antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

SYNPO Antibody

CATALOG NUMBER: 6049

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SYNPO antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SYNPO.
The immunogen is located within the last 50 amino acids of SYNPO.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SYNPO antibody can be used for detection of SYNPO by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of SYNPO are known to exist; this antibody will detect all three isoforms. SYNPO antibody is predicted to not cross-react with other SYNPO family member proteins.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
Predicted: 75, 99, 102 kDa
Observed: 95 kDa
Purification:
SYNPO Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SYNPO Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SYNPO antibody can be stored at 4°C , stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SYNPO
Additional Names:
SYNPO Antibody: KIAA1029, Synaptopodin
Protein Accession Number:
NP_001159680
Protein Gi Number:
261278297
Ncbi Gene Id Number:
11346
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SYNPO Antibody: SYNPO, also known as synaptopodin, is an actin-associated protein in telencephalic dendrites and renal podocytes. SYNPO is tightly associated with the dendritic spine apparatus, and mice lacking the SYNPO gene lack the apparatus. These SYNPO-null mice also demonstrate an impaired ability to express long-term potentiation as well as deficits in spatial memory tasks, indicating that SYNPO is involved in the regulation of synaptic plasticity. Recent studies suggest that SYNPO is linked to neuronal calcium stores and plays a role in the calcium store-associated ability of neurons to undergo long-term plasticity.
Background Reference 1:
Mundel P, Heid HW, Mundel TM, et al. Synaptopodin: an actin-associated protein in telencephalic dendrites and renal podocytes. J. Cell Biol. 1997; 139:193-204.
Background Reference 2:
Deller T, Merten T, Roth SU, et al. Actin-associated protein synaptopodin in the rat hippocampal formation: localization in the spine neck and close association with the spine apparatus of principal neurons. J. Compl. Neurol. 2000; 418:164-81.
Background Reference 3:
Deller T, Korte M, Chabanis S, et al. Synaptopodin-deficient mice lack a spine apparatus and show deficits in synaptic plasticity. Proc. Natl. Acad. Sci. USA 2003; 100:10494-9.
Background Reference 4:
Jedlicka P, Schwarzacher SW, Winkels R, et al. Impairment of in vivo theta-burst long-term potentiation and network excitability in the dentate gyrus of synaptopodin-deficient mice lacking the spine apparatus and the cisternal organelle. Hippocampus 2009; 19:130-40.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6049

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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