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Western blot analysis of ZIP1 in mouse kidney tissue lysate with ZIP1 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of ZIP1 in human kidney tissue with ZIP1 antibody at 2.5 μg/mL.
Immunofluorescence of ZIP1 in human kidney tissue with ZIP1 antibody at 20 μg/mL.
Western blot analysis of ZIP1 in mouse kidney tissue lysate with ZIP1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

ZIP1 Antibody

CATALOG NUMBER: 6081

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (88%)
Immunogen:
ZIP1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human ZIP1.
The immunogen is located within amino acids 40 - 90 of ZIP1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ZIP1 antibody can be used for detection of ZIP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of ZIP1 are known to exist; this antibody will detect both isoforms. ZIP1 antibody is predicted not to cross-react with other ZIP family proteins.
Positive Control 1:
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Predicted Molecular Weight:
Predicted: 13, 36 kDa
Observed: 38 kDa
Purification:
ZIP1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZIP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZIP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SLC39A1
Additional Names:
ZIP1 Antibody: ZIP1, ZIRTL, IRT1, ZIP1, CGI-08, CGI-71, Zinc transporter ZIP1, Solute carrier family 39 member 1, ZIP-1
Protein Accession Number:
NP_055252
Protein Gi Number:
21361423
Ncbi Gene Id Number:
27173
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZIP1 Antibody: ZIP1, also known as ZIRTL (zinc-iron regulated transporter-like), is the first mammalian member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. ZIP1 expression is markedly downregulated in a number of cancerous tissues and is thought to function as a tumor suppressor gene in prostate cancer. More recent studies have shown that overexpression of ZIP1 and concomitant increased levels of intracellular zinc in PC-3 cells cause a significant inhibition of NF-κB, leading to down-regulation of the antiapoptotic protein Bcl-2, Bcl-XL, and the apoptosis inhibitor XIAP, reducing the malignant potential of prostate cancer cells in vitro and in vivo.
Background Reference 1:
Lioumi M, Ferguson CA, Sharpe PT, et al. Isolation and characterization of human and mouse ZIRTL, a member of the IRT1 family of transporters, mapping within the epidermal differentiation complex. Genomics 1999; 62:272-80.
Background Reference 2:
Eide DJ. The SLC39 family of metal ion transporters. Pflugers Arch. 2004; 447:796-800.
Background Reference 3:
Taylor KM and Nicohlson RI. The LZT proteins; the LIV-1 subfamily of zinc transporters. Biochim. Biophys. Acta. 2003; 1611:16-30.
Background Reference 4:
Costello LC and Franklin RB. The clinical relevance of the metabolism of prostate cancer; zinc and tumor suppression: connecting the dots. Mol. Cancer. 2006; 5:17.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6081

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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