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Western blot analysis of ZIP8 in human spleen tissue lysate with ZIP8 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of ZIP8 in human spleen tissue with ZIP8 antibody at 5 μg/mL.
Immunofluorescence of ZIP8 in human spleen tissue with ZIP8 antibody at 20 μg/mL.
Western blot analysis of ZIP8 in human spleen tissue lysate with ZIP8 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

ZIP8 Antibody

CATALOG NUMBER: 6095

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (89%), Rat: (89%)
Immunogen:
ZIP8 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human ZIP8.
The immunogen is located within the last 50 amino acids of ZIP8.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ZIP8 antibody can be used for detection of ZIP8 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
ZIP8 antibody is predicted to not cross-react with other ZIP family members.
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Purification:
ZIP8 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZIP8 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZIP8 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Slc39a8
Additional Names:
ZIP8 Antibody: ZIP8, PP3105, BIGM103, LZT-Hs6, ZIP8, Zinc transporter ZIP8, BCG-induced integral membrane protein in monocyte clone 103 protein
Protein Accession Number:
NP_071437
Protein Gi Number:
205830403
Ncbi Gene Id Number:
64116
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZIP8 Antibody: The zinc transporter ZIP8, also known as SLC39A9, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. The zinc transporter family is divided into four subfamilies (I, II, LIV-1 and gufA). ZIP8 is glycosylated and located at the plasma membrane and mitochondria. It has been identified as the transporter responsible for transport of the toxic Cadmium cation. ZIP8 has also been suggested to play a role in the regulation of interferon-gamma expression in activated human T cells.
Background Reference 1:
Dufner-Beattie J, Langmade SJ, Wang F, et al. Structure, function, and regulation of a subfamily of mouse zinc transporter genes. J. Biol. Chem.2003; 278:50142-50.
Background Reference 2:
Eide DJ. The SLC39 family of metal ion transporters. Pflugers Arch.2004; 447:796-800.
Background Reference 3:
Taylor KM and Nicohlson RI. The LZT proteins; the LIV-1 subfamily of zinc transporters. Biochim. Biophys. Acta.2003; 1611:16-30.
Background Reference 4:
Dalton TP, He L, Wang B, et al. Identification of mouse SLC39A8 as the transporter responsible for cadmium-induced toxicity in the testis. Proc. Natl. Acad. Sci. USA2005; 102:3401-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6095

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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