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Western blot analysis of ZIP9 in HepG2 cell lysate with ZIP9 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of ZIP9 in human brain tissue with ZIP9 antibody at 2.5 μg/mL.
Immunofluorescence of ZIP9 in human brain tissue with ZIP9 antibody at 20 μg/mL.
Western blot analysis of ZIP9 in HepG2 cell lysate with ZIP9 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

ZIP9 Antibody

CATALOG NUMBER: 6097

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (89%)
Immunogen:
ZIP9 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human ZIP9.
The immunogen is located within amino acids 70 - 120 of ZIP9.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ZIP9 antibody can be used for detection of ZIP9 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
ZIP9 antibody is predicted to not cross-react with other ZIP family members.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
ZIP9 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZIP9 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZIP9 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Slc39a9
Additional Names:
ZIP9 Antibody: ZIP9, ZIP-9, ZIP9, UNQ714/PRO1377, Zinc transporter ZIP9, Solute carrier family 39 member 9
Protein Accession Number:
NP_060845
Protein Gi Number:
237874617
Ncbi Gene Id Number:
55334
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZIP9 Antibody: The zinc transporter ZIP9, also known as SLC39A9, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. The zinc transporter family is divided into four subfamilies (I, II, LIV-1 and gufA). ZIP9 is a multipass membrane protein that belongs to the ZIP transporter subfamily I. It is located at the trans-Golgi network regardless of zinc presence and is thought to be a zinc homeostasis regulator acting in the secretory pathway without significantly altering cytosolic zinc homeostasis.
Background Reference 1:
Dufner-Beattie J, Langmade SJ, Wang F, et al. Structure, function, and regulation of a subfamily of mouse zinc transporter genes. J. Biol. Chem.2003; 278:50142-50.
Background Reference 2:
Eide DJ. The SLC39 family of metal ion transporters. Pflugers Arch.2004; 447:796-800.
Background Reference 3:
Taylor KM and Nicohlson RI. The LZT proteins; the LIV-1 subfamily of zinc transporters. Biochim. Biophys. Acta.2003; 1611:16-30.
Background Reference 4:
Matsuura W, Yamazaki T, Yamaguchi-Iwai Y, et al. SLC39A9 (ZIP9) regulates zinc homeostasis in the secretory pathway: characterization of the ZIP subfamily protein in vertebrate cells. Biosci. Biotechnol. Biochem.2009; 73:1142-1148.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6097

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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