Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

<strong> Figure 1 WB Validation in Human, Mouse and Rat Tissues</strong><br>Loading: 10 μg of lysate Antibodies: ZIP14, 6105, 2 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Immunohistochemistry Validation of ZIP14 in Human Kidney</strong><br> Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ZIP14 antibody (6105) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 3 Immunohistochemistry Validation of ZIP14 in Mouse Colon</strong><br> Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-ZIP14 antibody (6105) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 4 Immunohistochemistry Validation of ZIP14 in Rat Kideny</strong><br> Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ZIP14 antibody (6105) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 1 WB Validation in Human, Mouse and Rat Tissues
Loading: 10 μg of lysate Antibodies: ZIP14, 6105, 2 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Trial Size

ZIP14 Antibody

CATALOG NUMBER: 6105

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
Anti-ZIP14 antibody (6105) was raised against a peptide corresponding to 16 amino acid synthetic peptide near the center of human ZIP14.

The immunogen is located within amino acids 230-280 of ZIP14.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
WB: 1-4 μg/mL; IHC-P: 1-2 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse, and rat samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of ZIP14 are known to exist; this antibody will detect both isoforms. ZIP14 antibody is predicted to not cross-react with other ZIP family members.
Positive Control 1:
Cat. No. 1304 – Human Liver Tissue Lysate
Positive Control 2:
Cat. No. 1405 - Mouse kidney Tissue Lysate
Positive Control 3:
Cat. No. 1465 - Rat kidney Tissue Lysate
Predicted Molecular Weight:
Predicted: 54 kDa
Observed: 52 kDa
Purification:
ZIP14 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZIP14 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZIP14 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Slc39a14
Additional Names:
ZIP14 Antibody: NET34, ZIP14, cig19, LZT-Hs4, KIAA0062, Zinc transporter ZIP14, LIV-1 subfamily of ZIP zinc transporter 4
Protein Accession Number:
NP_001128625
Protein Gi Number:
205830424
Ncbi Gene Id Number:
23516
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZIP14 Antibody: The zinc transporter ZIP14, also known as SLC39A14, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. The zinc transporter family is divided into four subfamilies (I, II, LIV-1 and gufA). ZIP14 is a glycosylated multipass plasma membrane protein that belongs to the ZIP transporter subfamily LIV-1. ZIP14 has been shown to contribute to the hypozincemia of inflammation and infection and is regulated in the liver by IL-6. In addition to zinc, ZIP14 is also involved in the cellular uptake of non-transferrin-bound iron as well as iron bound to transferrin.
Background Reference 1:
Dufner-Beattie J, Langmade SJ, Wang F, et al. Structure, function, and regulation of a subfamily of mouse zinc transporter genes. J. Biol. Chem. 2003; 278:50142-50.
Background Reference 2:
Eide DJ. The SLC39 family of metal ion transporters. Pflugers Arch. 2004; 447:796-800.
Background Reference 3:
Taylor KM and Nicohlson RI. The LZT proteins; the LIV-1 subfamily of zinc transporters. Biochim. Biophys. Acta. 2003; 1611:16-30.
Background Reference 4:
Taylor KM, Morgan HE, Johnson A, et al. Structure-function analysis of a novel member of the LIV-1 subfamily of zinc transporters, ZIP14. FEBS Lett. 2005; 579:427-32.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6105

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369