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Western blot analysis of ZBTB3 in rat brain tissue lysate with ZBTB3 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of ZBTB3 in human brain tissue with ZBTB3 antibody at 2.5 μg/mL.
Immunofluorescence of ZBTB3 in human brain tissue with ZBTB3 antibody at 20 μg/mL.
Western blot analysis of ZBTB3 in rat brain tissue lysate with ZBTB3 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

ZBTB3 Antibody

CATALOG NUMBER: 6119

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
ZBTB3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human ZBTB3.
The immunogen is located within amino acids 510 - 560 of ZBTB3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ZBTB3 antibody can be used for detection of ZBTB3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of ZBTB3 are known to exist. This antibody is predicted to not cross-react with other ZBTB protein family members.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
ZBTB3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZBTB3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZBTB3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZBTB3
Additional Names:
ZBTB3 Antibody: Zinc finger and BTB domain-containing protein 3
Protein Accession Number:
AAH25249
Protein Gi Number:
13376146
Ncbi Gene Id Number:
79842
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZBTB3 Antibody: The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. While little is known about ZBTB3, the related protein ZBTB2 is thought to be phosphorylated in response to the DNA damage, probably by either ATM or ATR. Other ZBTB proteins, such as ZBTB4 and ZBTB38 bind methylated DNA and repress transcription, suggesting that ZBTB3 may also act as a transcription repressor.
Background Reference 1:
Strausberg RL, Feingold EA, Grouse LH, et al. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc. Natl. Acad. Sci. USA2002; 99:16899-903.
Background Reference 2:
Matsuoka S, Ballif BA, Smogorzewska A, et al. ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. Science2007; 1160-1166.
Background Reference 3:
Filion GJP, Zhenilo S, Salozhin S, et al. A family of zinc finger proteins that bind methylated DNA and repress transcription. Mol. Cell. Biol.2006; 26:169-81.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6119

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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