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Western blot analysis of ZBTB5 in mouse brain tissue lysate with ZBTB5 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of ZBTB5 in human brain tissue with ZBTB5 antibody at 2.5 μg/mL.
Immunofluorescence of ZBTB5 in human brain tissue with ZBTB5 antibody at 20 μg/mL.
Western blot analysis of ZBTB5 in mouse brain tissue lysate with ZBTB5 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

ZBTB5 Antibody

CATALOG NUMBER: 6123

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
ZBTB5 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human ZBTB6.
The immunogen is located within the last 50 amino acids of ZBTB5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ZTBT5 antibody can be used for detection of ZTBT5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of ZBTB5 are known to exist; this antibody will recognize both isoforms. This antibody is predicted to not cross-react with other ZBTB protein family members.
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
ZBTB5 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZBTB5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZBTB5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZBTB5
Additional Names:
ZBTB5 Antibody: KIAA0354, Zinc finger and BTB domain-containing protein 5
Protein Accession Number:
NP_055687
Protein Gi Number:
7662074
Ncbi Gene Id Number:
9925
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZBTB5 Antibody: The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. ZBTB5 was identified though sequence analysis as a POZ domain Kruppel-like zinc finger (POK) protein. Further experiments indicated that it binds DNA and can directly repress transcription of the cell cycle arrest gene p21. ZBTB5 can also interact with co-repressor histone deacetylase complexes such as BCoR, NCoR, and SMRT via its POZ domain, resulting in deacetylation of histones Ac-H3 and Ac-H4 at the proximal promoter. ZBTB5 stimulates both cell proliferation and cell cycle progression, suggesting that it may act as a potential proto-oncogene.
Background Reference 1:
Strausberg RL, Feingold EA, Grouse LH, et al. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc. Natl. Acad. Sci. USA2002; 99:16899-903.
Background Reference 2:
Koh D, Choi W, Jeon B, et al. A novel POK family transcription factor, ZBTB5, represses transcription of p21CIP1 gene. J. Biol. Chem.2009; 284:19856-66.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6123

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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