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<strong>Figure 1. RHAMM KO Validation in HeLa Cells</strong><br>Loading: 10 μg of lysate Antibodies: XRHAMM 6189, 1 μg/mL and beta-actin 3779-1301, 1μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Western blot analysis of RHAMM in rat stomach tissue lysate with RHAMM antibody at 0.5 μg/mL.
Immunohistochemistry of RHAMM in human stomach tissue with RHAMM antibody at 2.5 μg/mL.
Immunofluorescence of RHAMM in human stomach tissue with RHAM antibody at 20 μg/mL.
Figure 1. RHAMM KO Validation in HeLa Cells
Loading: 10 μg of lysate Antibodies: XRHAMM 6189, 1 μg/mL and beta-actin 3779-1301, 1μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
KO
Trial Size

RHAMM Antibody

CATALOG NUMBER: 6189

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
RHAMM antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human RHAMM.
The immunogen is located within amino acids 80 - 130 of RHAMM.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
RHAMM antibody can be used for detection of RHAMM by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1475 - Rat Stomach Tissue Lysate
Positive Control 2:
Cat. No. 10-801 - Human Stomach Tissue Slide
Validation:

KO Validation (Figure 1): Anti-RHAMM antibodies (6189) specificity was further verified by RHAMM specific knockout. RHAMM signal was not detected in RHAMM knockout HeLa cells as compared to that in control wild type cells.

Purification:
RHAMM Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RHAMM Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RHAMM antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HMMR
Additional Names:
RHAMM Antibody: CD168, IHABP, RHAMM, Hyaluronan mediated motility receptor, Intracellular hyaluronic acid-binding protein
Protein Accession Number:
NP_036617
Protein Gi Number:
217272802
Ncbi Gene Id Number:
3161
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RHAMM Antibody: The hyaluronan-mediated motility receptor, also known as RHAMM, was initially identified as a soluble protein that could be released by sub-confluent migrating cells, promoting cell motility and invasion via interactions with hyaluronan (HA) and the cell surface. While RHAMM is normally poorly expressed in most normal tissues and is not required for embryonic development or normal cell homeostasis functions, its expression is increased during wound repair in response to hypoxia and fibrogenic factors. However, its overexpression is transforming in multiple types of cancers and is required for maintaining RAS transformation. RHAMM associates with BRCA1 and BARD1, attenuating the mitotic-spindle-promoting activity of RHAMM, which may contribute to tumor progression by promoting genomic instability.
Background Reference 1:
Hardwick C, Hoare K, Owens R, et al. Molecular cloning of a novel hyaluronan receptor that promotes tumor cell motility. J. Cell Biol. 1992; 117:1343-50.
Background Reference 2:
Samuel SK, Hurta RA, Spearman MA, et al. TGF-beta 1 stimulation of cell locomotion utilizes the hyaluronan receptor RHAMM and hyaluronan. J. Cell Biol. 1993; 123:749-58.
Background Reference 3:
Hall CL, Yang B, Yang X, et al. Overexpression of the hyaluronan receptor RHAMM is transforming and is also required for H-ras transformation. Cell 1995; 82:19-26.
Background Reference 4:
Maxwell CA, McCarthy J, and Turley E. Cell-surface and mitotic-spindle RHAMM: moonlighting or dual oncogenic functions? J. Cell Sci. 2008; 121:925-32.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6189

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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