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Western blot analysis of TM4SF1 in human lung tissue lysate with TM4SF1 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of TM4SF1 in human lung tissue with TM4SF1 antibody at 5 μg/mL.
Immunofluorescence of TM4SF1 in human lung tissue with TM4SF1 antibody at 20 μg/mL.
Immunohistochemistry of TM4SF1 in mouse lung tissue with TM4SF1 antibody at 5 μg/mL.
Immunofluorescence of TM4SF1 in mouse lung tissue with TM4SF1 antibody at 20 μg/mL.<br><br>Red: TM4SF1 Antibody (6229) <br> Blue: DAPI staining
Immunohistochemistry of TM4SF1 in mouse lung tissue with TM4SF1 antibody at 2 μg/mL.
Western blot analysis of TM4SF1 in human lung tissue lysate with TM4SF1 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

TM4SF1 Antibody

CATALOG NUMBER: 6229

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
TM4SF1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human TM4SF1.
The immunogen is located within the first 50 amino acids of TM4SF1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TM4SF1 antibody can be used for detection of TM4SF1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of TM4SF1 are known to exist; this antibody will recognize only the two largest isoforms. TM4SF1 antibody is predicted to not cross-react with other tetraspanin proteins.
Positive Control 1:
Cat. No. 1302 - Human Lung Tissue Lysate
Positive Control 2:
Cat. No. 10-101 - Human Lung Tissue Slide
Purification:
TM4SF1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TM4SF1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TM4SF1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TM4SF1
Additional Names:
TM4SF1 Antibody: L6, H-L6, M3S1, TAAL6, Transmembrane 4 L6 family member 1, Membrane component chromosome 3 surface marker 1
Protein Accession Number:
NP_055035
Protein Gi Number:
21265101
Ncbi Gene Id Number:
4071
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TM4SF1 Antibody: TM4SF1 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains and mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. TM4SF1 is a cell surface antigen and is highly expressed in the vascular endothelium of several human cancers. Knockdown experiments of TM4SF1 prevented filopodia formation, inhibited cell mobility, blocked cytokinesis, and inhibited maturation of VEGF-A-induced angiogenesis, suggesting that TM4SF1 may be an attractive target for anti-angiogenesis therapy.
Background Reference 1:
Marken JS, Schieven GL, Hellstrom I, et al. Cloning and expression of the tumor-associated antigen L6. Proc. Natl. Acad. Sci. USA 1992; 89:3503-7
Background Reference 2:
Romanska HM and Berditchevski F. Tetraspanins in human epithelial malignancies. J. Pathol. 2011; 223:4-14
Background Reference 3:
Shih SC, Zukauskas A, Li D, et al. The L6 TM4SF1 is critical for endothelial cell function and tumor angiogenesis. Cancer Res. 2009; 69:3272-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6229

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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