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Western blot analysis of ZFP219 in EL4 cell lysate with ZFP219 antibody at 1 μg/mL.
Immunohistochemistry of ZFP219 in mouse brain tissue with ZFP219 antibody at 5 μg/mL.
Immunofluorescence of ZFP219 in mouse brain tissue with ZFP219 antibody at 20 μg/mL.
Western blot analysis of ZFP219 in EL4 cell lysate with ZFP219 antibody at 1 μg/mL.
Trial Size

ZFP219 Antibody

CATALOG NUMBER: 6231

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
ZFP219 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human ZFP219.
The immunogen is located within amino acids 90 - 140 of ZFP219.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ZFP219 antibody can be used for detection of ZFP219 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of ZFP219 are known to exist; this antibody will recognize both.
Positive Control 1:
Cat. No. 1287 - EL4 Cell Lysate
Predicted Molecular Weight:
Predicted: 80 kDa
Observed: 78 kDa
Purification:
ZFP219 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZFP219 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZFP219 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZNF219
Additional Names:
ZFP219 Antibody: ZFP219, Zinc finger protein 219
Protein Accession Number:
NP_001095142
Protein Gi Number:
156415996
Ncbi Gene Id Number:
51222
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZFP219 Antibody: ZFP219 is a developmentally regulated member of the Kruppel-like zinc finger gene family that is thought to function as a transcriptional repressor. Yeast two-hybrid screening showed association with Sox9, a transcription factor that is essential for chondrogenesis. ZFP219 is specifically expressed in the developing limb buds and colocalizes with Sox9 in the nucleus. Knockdown of ZFP219 expression decreased Sox9-induced mRNA expression, and a dominant-negative mutant of ZFP219 inhibited Bmp2-induced chondrocyte differentiation, suggesting that ZFP219 plays an important role as a transcriptional partner of Sox9 in the regulation of chondrocyte differentiation.
Background Reference 1:
Sakai T, Toyoda A, Hashimoto K, et al. Isolation and characterization of a novel zinc finger gene, ZNF219, and mapping to the human chromosome 14q11 region. DNA Res. 2000; 7:137-41.
Background Reference 2:
Sakai T, Hino K, Wada S, et al. Identification of the DNA binding specificity of the human ZNF219 protein and its function as a transcriptional repressor. DNA Res. 2003; 10:155-65.
Background Reference 3:
Takigawa Y, Hata K, Muramatsu S, et al. The transcription factor Znf219 regulates chondrocyte differentiation by assembling a transcription factory with Sox9. J. Cell Sci. 2010; 123:3780-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6231

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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