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<strong>Figure 1 Western Blot Validation in Mouse 3T3 Cell Lysate</strong><br>Loading: 15 μg of lysates per lane.Antibodies: LIF 6245, (A: 1 μg/mL, B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation in Human Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: LIF 6245, (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation with Recombinant Protein</strong><br>Loading: 15 μg of lysates per lane.Antibodies: LIF 6245, (1: 1 μg/mL, 2: 2μg/mL, 3: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Western Blot Validation in Mouse Cell Lines and Tissue</strong><br>Loading: 15 μg of lysates per lane.Antibodies: LIF 6245, (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 Western Blot Validation in Rat Lung</strong><br>Loading: 15 μg of lysates per lane.Antibodies: LIF 6245, (1: 1 μg/mL, 2: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 6 Immunofluorescence Validation of LIF in Mouse 3T3 Cells</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed 3T3 cells labeling LIF with 6245 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Figure 1 Western Blot Validation in Mouse 3T3 Cell Lysate
Loading: 15 μg of lysates per lane.Antibodies: LIF 6245, (A: 1 μg/mL, B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Trial Size

LIF Antibody

CATALOG NUMBER: 6245

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
Anti-LIF antibody (6245) was raised against a peptide corresponding to 16 amino acids near the center of human LIF.
The immunogen is located within amino acids 50-100 of LIF.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
WB: 1-4 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of LIF are known to exist.
Positive Control 1:
Cat. No. 1287 - EL4 Cell Lysate
Positive Control 2:
Cat. No. 17-201 - 3T3/BALB Cell Slide
Predicted Molecular Weight:
Predicted: 22kD
Observed: 26kD (Post-modifications: N-linked Glycosylations)
Validation:

Recombinant Protein Test (Figure 3): Anit-LIF antibodies (6245) detected human LIF recombinant protein at different concentrations.

Isoforms:
Human LIF has 2 isoforms, including isoform 1 (202aa, 22kD) and isoform 2 (88aa, 9.6kD). Mouse LIF has 1 isoform (203aa, 22.3kD and Rat LIF also has 1 isoform (202aa, 22.1kD). 6245 can detect human isoform1 as well as mouse and rat LIF.
Purification:
LIF Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
LIF Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
LIF antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LIF
Additional Names:
LIF Antibody: CDF, DIA, HILDA, MLPLI, Leukemia inhibitory factor, Differentiation-stimulating factor, LIF
Protein Accession Number:
NP_002300
Protein Gi Number:
4504991
Ncbi Gene Id Number:
3976
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.
Background Reference 1:
Trouillas et al. Cytokine Netw. 2009; 20:51-62.
Background Reference 2:
Hilton et al. Anal. Biochem. 1988; 173:359-67.
Background Reference 3:
Schemlzer et al. Protein Expr. Purif. 1990; 1:54-62.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6245

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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