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Western blot analysis of DYRK2 in 293 cell lysate with DYRK2 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of DYRK2 in 293 cells with DYRK2 antibody at 10 μg/mL.
Immunofluorescence of DYRK2 in 293 cells with DYRK2 antibody at 20 μg/mL.
Western blot analysis of DYRK2 in 293 cell lysate with DYRK2 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

DYRK2 Antibody

CATALOG NUMBER: 6257

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (89%), Mouse: (89%)
Immunogen:
DYRK2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human DYRK2.
The immunogen is located within amino acids 520 - 570 of DYRK2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
DYRK2 antibody can be used for detection of DYRK2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
Two isoforms of DYRK2 are known to exist; this antibody will recognize both isoforms. DYRK2 antibody will not cross-react with other DYRK family members.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2:
Cat. No. 17-010 - HEK293 Cell Slide
Purification:
DYRK2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
DYRK2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
DYRK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DYRK2
Additional Names:
DYRK2 Antibody: Dual specificity tyrosine-phosphorylation-regulated kinase 2
Protein Accession Number:
NP_006473
Protein Gi Number:
4503427
Ncbi Gene Id Number:
8445
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DYRK2 Antibody: DYRK2 is a member of the dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family that is thought to be involved in cellular proliferation and apoptosis. Under normal conditions, nuclear but not cytoplasmic DYRK2 is ubiquitinated by MDM2, leading to its constitutive degradation. However, upon exposure to genotoxic stress, ATM phosphorylates DYRK2, leading to its dissociation from MDM2 and its phosphorylation of p53, thereby inducing apoptosis. Recent evidence also suggests that DYRK2 may serve as a scaffold that facilitates assembly of an E3 ubiquitin ligase.
Background Reference 1:
Becker W, Weber Y, Wetzel K, et al. Sequence characteristics, subcellular localization, and substrate specificity of DRYK-related kinases, a novel family of dual specificity protein kinases. J. Biol. Chem. 1998; 273:25893-902.
Background Reference 2:
Yoshida K. Role for DYRK family kinases on regulation of apoptosis. Biochem. Pharmacol. 2008; 76:1389-94.
Background Reference 3:
Taira N, Yamamoto H, Yamaguchi T, et al. ATM augments nuclear stabilization of DYRK2 by inhibiting MDM2 in the apoptotic response to DNA damage J. Biol. Chem. 2010; 285:4909-19.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6257

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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