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Western blot analysis of PRR5 in SK-N-SH cell lysate with PRR5 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
Immunohistochemistry of PPR5 in mouse brain tissue with PPR5 antibody at 5 μg/mL.
Immunofluorescence of PPR5 in mouse brain tissue with PPR5 antibody at 20 μg/mL.
Western blot analysis of PRR5 in SK-N-SH cell lysate with PRR5 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
Trial Size

PRR5 Antibody

CATALOG NUMBER: 6291

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PRR5 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human PRR5.
The immunogen is located within amino acids 310 - 360 of PRR5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
PRR5 antibody can be used for detection of PRR5 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
PRR5 antibody is predicted to not cross-react with other Protor protein family members.
Positive Control 1:
Cat. No. 1220 - SK-N-SH Cell Lysate
Purification:
PRR5 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PRR5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PRR5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PRR5
Additional Names:
PRR5 Antibody: PP610, PROTOR1, PROTOR-1, FLJ20185k, PP610, Proline-rich protein 5, Protein observed with Rictor-1, Protor-1
Protein Accession Number:
NP_851850
Protein Gi Number:
31317218
Ncbi Gene Id Number:
55615
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PRR5 Antibody: Proline-rich protein 5 (PRR5), also known as Protor-1, is a 388 amino acid protein in Protor family, is thought to act as a tumor suppressor in breast and colorectal tumorigenesis. PRR5 is widely expressed and possesses two RICTOR interaction sites and a C-terminal Proline rich region. It promotes Rapamycin complex 2 (mTORC2) activity. There are four isoforms of PRR5 that are produced as a result of alternative splicing events and these isoforms play an important role in the modulation of platelet-derived growth factor signaling and in the regulation of PDGFR-beta expression.
Background Reference 1:
Johnstone CN, Castellvi-Bel S, Chang LM, et al. PRR5 encodes a conserved proline-rich protein predominant in kidney: analysis of genomic organization, expression, and mutation status in breast and colorectal carcinomas. Genomics 2005; 85:338-51.
Background Reference 2:
Pearce LR, Huang X, Boudeau J, et al. Identification of Protor as a novel Rictor-binding component of mTOR complex-2. Biochem. J. 2007; 405:513-22.
Background Reference 3:
Woo SY, Kim DH, Jun CB, et al. PRR5, a novel component of mTOR complex 2, regulates platelet-derived growth factor receptor expression and signaling. J. Biol. Chem. 2007; 282:25604-12.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6291

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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