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Western blot analysis of ROCK2 in mouse brain tissue lysate with ROCK2 antibody at (A) 1 and (B) 2 μg/mL
Immunohistochemistry of ROCK2 in mouse brain tissue with ROCK2 antibody at 5 μg/mL.
Immunofluorescence of ROCK2 in mouse brain tissue with ROCK2 antibody at 20 μg/mL.
Western blot analysis of ROCK2 in mouse brain tissue lysate with ROCK2 antibody at (A) 1 and (B) 2 μg/mL
Trial Size

ROCK2 Antibody

CATALOG NUMBER: 6297

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (100%)
Immunogen:
ROCK2 antibody was raised against a 15 amino acid synthetic peptide near the center of human ROCK2.
The immunogen is located within amino acids 920 - 970 of ROCK2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ROCK2 antibody can be used for detection of ROCK2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
Three isoforms of ROCK2 are known to exist; this antibody recognizes all three isoforms. ROCK2 antibody is predicted to not cross-react with other ROCK protein family members.
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 153 kDa
Observed: 160 kDa
Purification:
ROCK2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ROCK2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ROCK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ROCK2
Additional Names:
ROCK2 Antibody: ROCK-II, KIAA0619, Rho-associated protein kinase 2, Rho kinase 2, ROCK-II
Protein Accession Number:
NP_004841
Protein Gi Number:
41872583
Ncbi Gene Id Number:
9475
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ROCK2 Antibody: ROCK2 is a member of the AGC serine/threonine protein kinase family that regulates the assembly of the actin cytoskeleton. ROCK2 contains a protein kinase domain, a coiled-coil region and a zinc finger region and appears to be present as a dimer. ROCK2, like its isozyme ROCK1, is a downstream target of the small G-protein Rho and plays a role in smooth muscle contraction, malignant cell transformation, tumor invasion and metastasis, and mediates the cellular morphological changes during apoptosis.
Background Reference 1:
Leung T, Chen XQ, Manser E, et al. The p160 RhoA-binding kinase ROK alpha is a member of a kinase family and is involved in the reorganization of the cytoskeleton. Mol. Cell Biol. 1996; 16:5313-27.
Background Reference 2:
Takahashi N, Tuiki H, Saya H, et al. Localization of the gene coding for ROCK II/Rho kinase on human chromosome 2p24. Genomics 1999; 55:235-7
Background Reference 3:
Ishizaki T, Maekawa M, Fujisawa K, et al. The small GTP-binding protein Rho binds to and activates a 160 kDa Ser/Thr protein kinase homologous to myotonic dystrophy kinase. EMBO J. 1996; 15:1885-93.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6297

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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