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Western blot analysis of SIK2 in SW480 cell lysate with SIK2 antibody at 1 μg/mL.
Immunocytochemistry of SIK2 in SW480 cells with SIK2 antibody at 2.5 μg/ml.
Immunofluorescence of SIK2 in SW480 cells with SIK2 antibody at 5 μg/ml.
Western blot analysis of SIK2 in SW480 cell lysate with SIK2 antibody at 1 μg/mL.
Trial Size

SIK2 Antibody

CATALOG NUMBER: 6301

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SIK2 antibody was raised against a 16 amino acid synthetic peptide near the center of human SIK2.
The immunogen is located within amino acids 270 - 320 of SIK2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
SIK2 antibody can be used for detection of SIK2 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
SIK2 antibody is predicted to not cross-react with other SIK protein family members.
Positive Control 1:
Cat. No. 1221 - SW480 Cell Lysate
Purification:
SIK2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SIK2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SIK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SIK2
Additional Names:
SIK2 Antibody: QIK, SNF1LK2, LOH11CR1I, KIAA0781, QIK, Qin-induced kinase, SIK-2
Protein Accession Number:
EAW67148
Protein Gi Number:
38569460
Ncbi Gene Id Number:
23235
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SIK2 Antibody: Salt-inducible kinase 2 (SIK2), like its closely related homolog SIK1, belongs AMPK subfamily of the Ser/Thr protein kinase family and negatively regulates CRE-binding protein (CREB) activity by phosphorylating the CREB-specific coactivator transducer of regulated CREB activity (TORC). SIK2 is thought to be part of a signaling cascade that regulates the expression and activity of the insulin-induced genes PGC-1 alpha and UCP-1 in brown adipocytes, impairment of which has been implicated in obesity and insulin resistance in human and animal models. SIK2 has also been reported as a key regulator for neuronal survival after ischemia, suppressing CREB-mediated gene expression after oxygen-glucose deprivation.
Background Reference 1:
Horike N, Takemori H, Katoh Y, et al. Adipose-specific expression, phosphorylation of Ser794 in insulin receptor substrate-1, and activation in diabetic animals of salt-inducible kinase-2. J. Biol. Chem. 2003; 278:18440-7.
Background Reference 2:
Screaton RA, Conkright MD, Katoh Y, et al. The CREB coactivator TORC2 functions as a calcium- and cAMP-sensitive coincidence detector. Cell 2004; 119:61-74.
Background Reference 3:
Muraoka M, Fukushima A, Viengchareun S, et al. Involvement of SIK2/TORC2 signaling cascade in the regulation of insulin-induced PGC-1alpha and UCP-1 gene expression in brown adipocytes. Am. J. Physiol. Endocrinol. Metab. 2009; 296:E1430-9.
Background Reference 4:
Sasaki T, Takemori H, Yagita Y, et al. SIK2 is a key regulator for neuronal survival after ischemia via TORC1-CREB. Neuron 2011; 69:106-19.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6301

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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