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Western blot analysis of IRF4 in Ramos cell lysate with IRF4 antibody at 0.5 μg/mL.
Immunocytochemistry of IRF4 in Jurkat cells with IRF4 antibody at 10 μg/mL.
Immunofluorescence of IRF4 in Jurkat cells with IRF4 antibody at 20 μg/mL.
Western blot analysis of IRF4 in Ramos cell lysate with IRF4 antibody at 0.5 μg/mL.
Trial Size

IRF4 Antibody

CATALOG NUMBER: 6307

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
IRF4 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human IRF4.
The immunogen is located within the last 50 amino acids of IRF4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
IRF4 antibody can be used for detection of IRF4 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
IRF4 antibody is predicted to not cross-react with other IRF protein family members. IRF4 often migrates at a higher than predicted molecular weight in SDS-PAGE.
Positive Control 1:
Cat. No. 1225 - Ramos Cell Lysate
Positive Control 2:
Cat. No. 17-005 - Jurkat Cell Slide
Purification:
IRF4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IRF4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IRF4 antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IRF4
Additional Names:
IRF4 Antibody: MUM1, LSIRF, NF-EM5, MUM1, Interferon regulatory factor 4, Lymphocyte-specific interferon regulatory factor, IRF-4
Protein Accession Number:
NP_002451
Protein Gi Number:
167555104
Ncbi Gene Id Number:
3662
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IRF4 Antibody: Interferons (IFNs) are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. Another member, IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. IRF4 expression is tightly regulated in resting primary T cells and plays an essential role in the homeostasis and function of mature lymphocytes. IRF4 is induced by Toll-like receptor (TLR) activation and acts as a negative regulator of TLR signaling.
Background Reference 1:
Malmgaard L. Induction and regulation of IFNs during viral infections. J. Interferon & Cyto. Res. 2004; 24:439-54
Background Reference 2:
Sato M, Suemori H, Hata N, et al. Distinct and essential roles of transcription factors IRF-3 and IRF-7 in response to viruses for IFN-alpha/beta gene induction. Immunity 2000; 13:539-48.
Background Reference 3:
Mittrucker HW, Matsuyama T, Grossman A, et al. Requirement for the transcription factor LSIRF/IRF4 for mature B and T lymphocyte function. Science 1997; 275:540-3.
Background Reference 4:
Negishi H, Ohba Y, Yanai H, et al. Negative regulation of Toll-like-receptor signaling by IRF4. Proc. Natl. Acad. Sci. USA 2005; 102:15989-94

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6307

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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