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Western blot analysis of TYW2 in K562 cell lysate with TYW2 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of TYW2 in K562 cells with TYW2 antibody at 10 μg/mL.
Western blot analysis of TYW2 in K562 cell lysate with TYW2 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

TYW2 Antibody

CATALOG NUMBER: 6375

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (74%)
Immunogen:
TYW2 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TYW2.
The immunogen is located within the first 50 amino acids of TYW2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
TYW2 antibody can be used for detection of TYW2 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Specificity:
TYW2 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW2 are known to exist; this antibody will detect both isoforms
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 2:
Cat. No. 17-004 - K-562 Cell Slide
Purification:
TYW2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TYW2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TYW2 antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TRMT12
Additional Names:
TYW2 Antibody: TYW2, TRM12, TYW2, tRNA wybutosine-synthesizing protein 2 homolog, Alpha-amino-alpha-carboxypropyl transferase TYW2, tRNA-yW-synthesizing protein 2
Protein Accession Number:
Q53H54
Protein Gi Number:
157388919
Ncbi Gene Id Number:
55039
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TYW2 Antibody: TYW2, also known as TRM12, is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW2 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation. TYW2 was found to be amplified in 7 of 8 breast cancers cell lines analyzed, and qPCR analysis of 30 breast tumors showed overexpression of TYW2 mRNA of over 2-fold in 26, suggesting that TYW2 may play a role in breast cancer.
Background Reference 1:
Noma A and Suzuki T. Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis. Nucleic Acids Symp. Ser. (Oxf) 2006; 65-6
Background Reference 2:
Noma A, Kirino Y, Ikeuchi Y, et al. Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA. EMBO J. 2006; 25:2142-54
Background Reference 3:
Rodriquez V, Chen Y, Elkahloun A, et al. Chromosome 8 BAC array comparative genomic hybridization and expression analysis identify amplification and overexpression of TRMT12 in breast cancer. Genes Chromosomes Cancer 2007; 46:694-707

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6375

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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