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Western blot analysis of ATAD3A in Daudi cell lysate with ATAD3A antibody at 1 μg/mL .
Immunocytochemistry of ATAD3A in Daudi cells with ATAD3A antibody at 20 μg/mL.
Immunofluorescence of ATAD3A in Daudi cells with ATAD3A antibody at 20 μg/mL.
Western blot analysis of ATAD3A in Daudi cell lysate with ATAD3A antibody at 1 μg/mL .
Trial Size

ATAD3A Antibody

CATALOG NUMBER: 6387

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
ATAD3A antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human ATAD3A.
The immunogen is located within amino acids 110 - 160 of ATAD3A.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
ATAD3A antibody can be used for detection of ATAD3A by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
ATAD3A antibody is predicted to not cross-react with other ATAD protein family members. At least two isoforms are known to exist.
Positive Control 1:
Cat. No. 1224 - Daudi Cell Lysate
Purification:
ATAD3A Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ATAD3A Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ATAD3A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ATAD3A
Additional Names:
ATAD3A Antibody: ATPase family AAA domain-containing protein 3A
Protein Accession Number:
Q9NVI7
Protein Gi Number:
283436220
Ncbi Gene Id Number:
55210
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ATAD3A Antibody: ATAD3A is a member of the AAA ATPase family, a family of proteins that catalyze ATP into ADP and are involved in several cellular functions such as cell-cycle regulation, protein proteolysis and transport. The AAA ATPase family is characterized by a highly conserved AAA motif containing Walker homology sequences and imparting ATPase activity. Mitochondrial membrane proteins ATAD3A/B contribute to the stabilization of nucleoids and may participate in the transformation pathway and the chemosensitivity of oligodendrogliomas. The gene encoding ATAD3A/B/C maps to human chromosome 1 and has been suggested to be an anti-apoptotic marker.
Background Reference 1:
Patel S and Latterich M. The AAA team: related ATPases with diverse functions. Trends Cell Biol. 1998; 8:65-71.
Background Reference 2:
Ogura T and Wilkinson AJ. AAA+ superfamily ATPases: common structure—diverse function. Genes Cells 2001; 6:575-97.
Background Reference 3:
Hubstenberger A, Labourdette G, Baudier J, et al. ATAD3A and ATAD3B are distal 1p-located genes differentially expressed in human glioma cell lines and present in vitro anti-oncogenic and chemoresistant properties. Exp. Cell Res. 2008; 314:2870-83.
Background Reference 4:
Fang HY, Chang CL, Hsu SH, et al. ATPase family AAA domain-containing 3A is a novel anti-apoptotic factor in lung adenocarcinoma cells. J. Cell Sci. 2010; 123:1171-80.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6387

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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