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Western blot analysis of SPRYD5 in rat brain tissue lysate with SPRYD5 antibody at (A) 0.25 and (B) 0.5 μg/mL .
Immunohistochemistry of SPRYD5 in mouse brain tissue with SPRYD5 antibody at 5 μg/mL.
Immunofluorescence of SPRYD5 in mouse brain tissue with SPRYD5 antibody at 20 μg/mL.
Western blot analysis of SPRYD5 in rat brain tissue lysate with SPRYD5 antibody at (A) 0.25 and (B) 0.5 μg/mL .
Trial Size

SPRYD5 Antibody

CATALOG NUMBER: 6409

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SPRYD5 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SPRYD5.
The immunogen is located within amino acids 350 - 400 of SPRYD5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SPRYD5 antibody can be used for detection of SPRYD5 by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
SPRYD5 antibody is predicted to not cross-react with other SPRYD protein family members. At least two isoforms of SPRYD5 are known to exist; this antibody will detect both isoforms
Positive Control 1:
Cat. No. 1401 - Mouse Heart Tissue Lysate
Purification:
SPRYD5 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SPRYD5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SPRYD5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TRIM51
Additional Names:
SPRYD5 Antibody: SPRYD5, TRIM51A, SPRYD5, Tripartite motif-containing protein 51, SPRY domain-containing protein 5
Protein Accession Number:
NP_116070
Protein Gi Number:
209862805
Ncbi Gene Id Number:
84767
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SPRYD5 Antibody: The SPRY domain-containing protein 5 (SPRYD5) is a member of a family of proteins whose sole common characteristic is the presence of a SPRY domain. SPRY domains are structural domains that were first described in the fungal Dictyostelium discoideum tyrosine kinase spore lysis A. In most systems SPRY domains provide binding sites for regulatory proteins or intramolecular binding sites that maintain the structural integrity of a protein. SPRYD5 belongs to the TRIM/RBCC family and contains one B box-type zinc finger, one B30.2/SPRY domain and one RING-type zinc finger. Little is known of the function of the SPRYD5 protein.
Background Reference 1:
Tae H, Casarotto MG, and Dulhunty AF. Ubiquitous SPRY domains and their role in the skeletal type ryanodine receptor. Eur. Biophys. J. 2009; 39:51-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6409

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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